Expression And Clinical Signifcance Of Serum SCD14 And IL-8 In Patients With Primary Biliary Cholangitis

Objective:To explore the clinical values of serum soluble CD14 and interleukin-8 in patients with primary biliary cholangitisMethods:A total of 55 patients with PBC and 15 healthy controls were studied,including 45 ursodesoxycholic acid(UDCA)treatment-naive patients with PBC(chronic hepatitis stage,compensatory stage of cirrhosis,and decompensated stage of cirrhosis,with 15 people in each group)and 10 patients with PBC complicated with primary liver carcinoma(PLC).Clinical data and serum samples of all subjects were collected.The levels of sCD14 and IL-8 in secrum were measured by enzyme linked immunosorbent assay(ELISA),and their level changes in PBC disease progression were analyzed.The receiver operating characteristic(ROC)curve was used to evaluate the value of serum sCD14 and IL-8 in diagnosis of PBC complicated with PLC.When treatment-naive patients had been treated with UDCA for 6 months,the response rate of UDCA was evaluated according to Paris-I criteria,and the relationship between baseline serum sCD14,IL-8 and UCDA response rate was analyzedResults:1.The serum sCD14 levels of patients with PBC in chronic hepatitis[(17.41 ± 1.70)ug/ml],compensatory stage of cirrhosis[(19.17±1.01)ug/ml],decompensated stage of cirrhosis[(21.99±0.79)ug/ml]and patients with PBC complicated with PLC[(23.84±2.21)ug/ml]increased gradually,and were significantly higher than those of the control group[(9.60±0.94)ug/ml](P<0.05).There were statistical difference between each group(P<0.01).The serum sCD14 level[(21.0±1.4)ug/ml]of PBC patients with positive anti-gp210 antibody was significantly higher than those with negative anti-gp210[(19.2±2.3)ug/ml](P<0.05)2.The serum IL-8 levels of patients with PBC in chronic hepatitis[(272.27±23.31)pg/ml],compensatory stage of cirrhosis[(317.38±18.44)pg/ml],and decompensated stage of cirrhosis[(349.20±16.56)pg/ml]and patients with PBC complicated with PLC[(378.30±18.57)pg/ml]increased gradually,and were significantly higher than those of the control group[(154.54±10.88)pg/ml](P<0.05).There were statistical difference between each group(P<0.01).The serum IL-8 level[(340.0±19.8)pg/ml]of PBC patients with positive anti-gp210 antibody was significantly higher than those with negative anti-gp210[(306.5±34.5)pg/ml](P<0.05)3.In treatment-naive patients with PBC,serum sCD 14 was positively correlated with serum IL-8(r=0.840,P<0.01).Serum sCD14 was positively correlated with gamma glutamyl transferase(GGT),immunoglobulin G(IgG),liver stiffness measurement(LSM)and Mayo scores(r=0.3 82,0.593,0.345,0.675,P<0.05).Serum IL-8 was positively correlated with GGT,IgG,LSM and Mayo scores(r=0.495,0.663,0.340,0.762,P<0.05)4.The area under the curve(AUC)of serum sCD14 in diagnosis of PBC complicated with PLC was 0.769(95%CI 0.606～0.932),and the critical value was 20.53ug/ml.The sensitivity and specificity were 80.0%and 68.9%respectively.The AUC of serum IL-8 in diagnosis of PBC complicated with PLC was 0.787(95%CI 0.642～0.931),and the critical value was 325.43pg/ml.The sensitivity and specificity were 90.0%and 60.0%respectively5.Among the 45 treatment-naive patients with PBC,27 patients had a good response,while 18 patients had a poor response.The baseline serum levels of sCD14 and IL-8 in the patients with poor response[(20.8±1.7)ug/ml,(329.9±28.8)pg/ml]were significantly higher than those with good response[(18.7±2.2)ug/ml,(302.0±34.2)pg/ml],and the difference was statistically significant between the two groups(P<0.01)Conclusion:1.In patients with primary biliary cholangitis,the serum levels of soluble CD14 and interleukin-8 increased gradually with the progress of liver inflammation and liver fibrosis,and the relationship between serum sCD14 and IL-8 were positively correlated2.In patients with primary biliary cholangitis,serum soluble CD 14 and interleukin-8 may be used as serological markers for the diagnosis of PBC complicated with primary liver carcinoma3.In patients with primary biliary cholangitis,the serum levels of soluble CD14 and interleukin-8 at baseline had a certain predictive value for the biochemical response rate of ursodesoxycholic acid.As the level increased,the response rate decreased.