Antitumor Activity Of TMPyP4 On Human Glioma Cells

Objective: DNA G-quadruplexes are four-stranded complanate nucleic acid secondary structures formed in specific G-rich sequences,which play important roles in DNA replication,gene expression and telomere maintenance.However,its biological relevance has not been fully understood.In cancer cells,telomerase reactivation contributes to the limitless proliferation potential and chemoradiotherapy resistance.Mutations within the telomerase promoter region have occurred in many types of cancers,including approximately 80% primary glioblastoma containing these mutations,which combined with IDH mutation.We have presented evidence that G-rich sequences within TERT mutation region can adopt a parallel G4 structure;mutation within this region will decrease the stability and inhibit G4-mediated transcriptional silencing effects,resulting increased expression and activation of telomerase,which maybe the important course for telomerase reactivation in cancer cells.TMPy P4 is a ligand of G-quadruplex,which can stabilize the G-quadruplex structure in the promote.This study aims to analyze the effect of TMPy P4 on T98 G and U251 cells,which will provide a new therapy on cancer targeting treatment.Methods: We use CCK-8 assay to analyze the proliferation of T98 G and U251.The flow cytometry was performed to analyze cell apoptosis.Results: U251 and T98 G cells had not been effected by TMPy P4 when the concentration less than 50 m M.High concentration significant effects the proliferation of both cells.High concentration of TMPy P4 makes minor cell apoptosis happened.Conclusion:TMPy P4 can inhibit the U251 and T98 G cells proliferation,and induce minor cell apoptosis.