MiR-181c Suppresses Growth And Metastasis Of Hepatocellular Carcinoma By Modulating NCAPG

Objective: In this study,we investigated the expression of miR-181 c and NCAPG(non-SMC condensin I complex subunit G)in HCC,and their effects on the biological function of HCC cells,as well as the association between miR-181 c and NCAPG.Methods: The database was used to investigate the expression of miR-181 c and NCAPG in HCC tissues and normal liver tissues.quantitative real-time polymerase chain reaction(qRT-PCR)was used to investigate the expression of miR-181 c and NCAPG mRNA in 52 HCC tissues and corresponding adjacent tissues,and then we investigated the correlation between miR-181 c and NCAPG expression levels.Highand low-expression miR-181 c cell models were constructed in the HCC cell lines SMMC-7721 and MHCC-97 H.The effects of miR-181 c on the migration and invasion of HCC cells were investigated via cell wound-healing and Transwell.The effect of miR-181 c on HCC cell apoptosis was investigated via flow cytometry.The effect of miR-181 c on the proliferation of HCC cells was analyzed via 5-ethynyl-2’-deoxyuridine(EdU)proliferation assay.A nude mouse xenograft model was constructed to investigate the effect of miR-181 c on the growth and metastasis of HCC cells in vivo.Western blot was used to investigated the relationship between miR-181 c and NCAPG.The luciferase reporter gene assay was performed to verify whether NCAPG is the target gene of miR-181 c.Results: In HCC tissues,downregulation of miR-181 c was apparent compared with non-tumorous adjacent tissues.There was a negative correlation between miR-181 c expression and NCAPG in HCC tissue samples.Expression of miR-181 c in HCC cells was also significantly lower than it was in normal human liver cells.miR-181 c inhibits the migration,invasion and proliferation of HCC cells,and promotes apoptosis.miR-181 c inhibits tumor growth and metastasis in vivo.miR-181 c inhibits NCAPG expression in HCC cells.Dual luciferase reporter gene assay revealed that NCAPG is the target gene of miR-181 c.Conclusion: Our findings demonstrate that miR-181 c inhibits the growth and metastasis of hepatocellular carcinoma via targeting NCAPG.Thus,our study indicates that miR-181 c play critical roles in the development and progression of HCC,suggesting that miR-181 c may be novel potential therapeutic targets and new ideas for patients with HCC.