Clinical Features And Differential Gene Screening Of Invasive Behaviors Between Glioma And Brain Metastasis

Objective: To analyze the clinical features of invasive behaviors between glioma and metastatic brain tumor,and using bioinformatics strategies and techniques to screen invasive differentially expressed genes.Methods: Patients with glioma or brain metastases diagnosed pathologically from November 2017 to November 2019 in the Affiliated Hospital were selected.A total of 52 clinical data and tumor specimens were collected and divided into low-grade glioma groups(A Group,n = 19),high-grade glioma group(group B,n = 18),and metastatic tumor group(group C,n = 15),according to pathological diagnosis.According to the follow-up results,the survival period of surviving patients was determined.The clinical characteristics were retrospectively analyzed to make Kaplan-Meier survival curve.Glioma and brain metastasis tissue samples were obtained for RNA sequencing.Differentially expressed genes were preliminary screened.After GO analysis and biological enrichment,KEGG pathway annotation,Pub Med literature search,and Gene Card gene tissue location query,invasive differentially expressed genes were selected.Then,spreaman correlation analysis between differential genes and pathological grade was performed.Real-time fluorescence quantitative polymerase chain reaction technology was used to verify the expression level of invasive genes in tumors.Results: There was no statistically significant difference in the baseline data of groups A,B,and C in gender,age,and surgical approach.The median survival time of patients in group A was 12 months,the median survival time of patients in group B was 5 months,and the median survival time of patients in group C was 10 months.The survival curve found that the clinical characteristics of tumor invasive behavior include: tumor size,edema range,tumor blood supply,vascular invasion,tumor grade,and Ki 67 expression.1083 differentially expressed genes were obtained by RNA sequencing,including 585 upregulation and 498 down-regulation.According to GO and KEGG screening,66 invasive differentially expressed genes were obtained,of which 48 were up-regulated and 18 were down-regulated.Via Pub Med screening,34 invasive genes have not been reported,including 23 up-regulated and 11 down-regulated.Gene Card screened the invasion genes CALM3,CAMK2 A,CAMK2B,PRKCG whose expression level in the central nervous system was more than twice that of other systems.Theoretical analysis found that the expression levels of CALM3,CAMK2 A,CAMK2B,and PRKCG were negatively correlated with pathological grade(P < 0.01),of which CALM3 expression was highly correlated with brain metastasis(P < 0.01).CALM3 m RNA was significantly down-regulated in brain metastasis(P < 0.05),PRKCG m RNA was significantly down-regulated in both glioma and brain metastasis P < 0.05),but there was no significant change in the expression of CAMK2 A m RNA and CAMK2 B m RNA.Conclusion:(1)Clinical invasive behaviors of glioma and brain metastasis such as upregulated Ki67,tumor enlargement,increased edema,vascular invasion,and increased pathological grade all indicate a poor prognosis.(2)This study successfully screened the invasive behavioral genes CALM3 and PRKCG of gliomas and brain metastasis.(3)The down-regulation of CALM3 has a higher correlation with the aggressive behavior of brain metastasis,while the down-regulation of PRKCG plays a role in the aggressive behavior of glioma and brain metastasis.(4)Detection of CALM3 and PRKCG may be helpful in evaluating aggressive behavior and provide a reference for the targeted therapy in glioma and brain metastasis.