Effects Of Glycyrrhiza Polysaccharide-iron On The Solubility And Stability Of 14 Kinds Of Chinese Medicine Monomer Components And Effective Components Of Paeonia Lactiflora Pall

Glycyrrhiza polysaccharide（GPS）is one of the active ingredients in licorice and has a variety of pharmacological activities.It can protect the cardiovascular,regulate immunity,but also has the advantages of high efficiency,low toxicity and no cytotoxicity.In recent years,studies have found that polysaccharides can also reduce the toxicity of small molecule compounds,as natural stabilizers for other active ingredients,solubilizers and natural drug carriers.There have been more and more reports on the biological activity of GPS,but there are few reports on the effects of metal ions on the role of GPS in the compatibility of traditional Chinese medicine（TCM）.Therefore,this experiment takes glycyrrhizin polysaccharide-Iron（GPS-I）as the model and takes the biological monomer component as the object to study,so as to provide a reference for the compatibility of TCM and the control of metal ions during the decoction process.In order to study the effect of Fe³⁺of GPS-I on the function of GPS in the compatibility of TCM,this study selected flavonoids and organic acid,a total of 14kinds of TCM monomer components and active ingredients in Paeonia lactiflora Pall.as the research objects.Through the preparation of GPS-I,from the perspective of in vitro biopharmaceutics,based on the solubility and stability of Chinese medicine monomer components,this experiment studied the effect of Fe³⁺of GPS-I on the compatibility of GPS,in order to provide some reference for the compatibility of TCM and the control of metal ions during the boiling process.1.Preparation and study its physical and chemical properties of GPS-I.The study determined the temperature of the reaction solution,the amount of Fe Cl₃ added,and the p H of the reaction,and screened the best preparation process of GPS-I.The best preparation process conditions were 60℃,2 mol/L Fe Cl₃ 3 m L,p H=8.The content of Fe³⁺in GPS-I was detected Ultraviolet-visible spectrophotometry（UV）.The general physical and chemical properties of GPS-I were studied,and the structure of GPS-I was characterized by infrared spectrophotometry（IR）.Finally,the antioxidant activity in vitro was studied.The iron content in GPS-I prepared by o-phenanthroline spectrophotometry was 14.44%.GPS-I is brown-red powder,odorless and tasteless,soluble in water.Aqueous solution is red-brown transparent liquid,weakly alkaline,and insoluble in organic solvents such as ethanol and acetone,The IR results showed that the absorption of GPS-I at 912 cm^-1 was the characteristic absorption peak ofβ-Fe OOH,indicating that the iron core of it exists in a polymerizedβ-Fe OOH structure.GPS and Fe³⁺may be due to the OH-in GPS replaces the outer water molecules of Fe³⁺to complex with Fe³⁺.The complexation of the two does not fundamentally change the structure of GPS,and still retains the original characteristic absorption peak.The results of the DPPH free radical scavenging test indicated that GPS-I had a weak scavenging ability for DPPH free radicals.The presence of Fe³⁺had a greater effect on the antioxidant activity of GPS,which significantly reduced the antioxidant activity of GPS.2.The effects of GPS and GPS-I on the solubility of 14 kinds of flavonoids and organic acids in water and the stability in 80℃/PH 9 buffer solution were studied by HPLC.Equilibrium solubility method was used to explore the influence of the two on the solubility of TCM monomer components,and visual analysis and principal component analysis were used to comprehensively analyze the solubility results.The results showed that after adding GPS,the solubility of 14 monomer components increased to varying degrees.After adding GPS-I,the solubility of the monomer components showed different degrees of decrease compared to GPS.This showed that Fe³⁺of GPS-I had an adverse effect on the solubilization of GPS.The constant temperature acceleration method was used to study the effects of GPS and GPS-I on the stability of 14 kinds of monomer components.The results showed that under the experimental conditions,GPS had a stabilizing effect on 14 kinds of monomer components,and GPS-I would accelerate the degradation of the monomer components,indicating that Fe³⁺of GPS-I had an adverse effect on GPS’s stabilizing effect.3.Based on the above research results,further studies the effects of GPS and GPS-I on the solubility in water and the stability under high temperature and simulated gastrointestinal conditions of the active ingredients of Paeonia lactiflora Pall.The effect of the solubility of the 4 common active ingredients was studied.The results showed that the solubility of the active ingredients was reduced to varying degrees after adding GPS-I,and Fe³⁺of GPS-I had an adverse effect on the solubilization of GPS.The results of the study on the influence of the stability of 7 common active ingredients showed that GPS would increase the stability of the active ingredients.under high temperature conditions,Fe³⁺of GPS-I was not conducive to the stability of the active ingredients.Under simulated gastrointestinal conditions,the 7 effective ingredients were relatively stable,and GPS had a slight stabilizing effect,GPS-I had obvious effects on benzoyl paeoniflorin,ethyl gallate and catechin,which would accelerate their degradation rate.Through the study of this subject,it is shown that GPS has a solubilizing and stabilizing effect on the flavonoids,organic acids and active ingredients in Paeonia lactiflora Pall.Fe³⁺of GPS-I has an adverse effect on the solubilization and stabilization of GPS.Polysaccharides should avoid contact with metal ions as much as possible in the use of TCM,and metal ions need to be properly controlled during the cooking process.