Study On Soil Remediation And Degradation Mechanism Of Fomesafen-degrading Bacteria

For the study of bioremediation of fomesafen,a hot topic in the research at present.Examples are rarely reported that degrading strain repair fomesafen successful,because the environmental climate,the soil and degradation characteristics of degradation bacteria in different parts of area,its has different ability of degrading bacteria,degradation bacteria will also be different.Often,the phenomenon of the pollution of soil degradation effect large differences.In order to solve non indigenous strain in the low survival rate,low degradation activity and long delay time in the bioremediation,according to the current research of Heilongjiang province.In this study,fomesafen is tested pesticides,by enrichment culture for Efficient Degradation Strain FB5 basis in the early,the strains were applied to the fomesafen residues in soil,bioremediation,explore the effect of soil bioremediation of the strain,the strain specific effects of soil on the indigenous microbial population dynamics.In this study,fomesafen is tested pesticides,by enrichment culture for Efficient Degradation Strain FB5 basis in the early,the strains were applied to the fomesafen residues in soil,explored the effect of soil bioremediation of the strain,specified effects of the strain soil on the indigenous microbial population dynamics.For the further development of this kind of strain and soil remediation provide theoretical guidance.In order to clear the strain in fomesafen about metabolic mechanism and molecular mechanism.In this paper,the way of GS-MS and proteomics study on system in the direction,to clarify the degradation pathway of degradation bacteria,to clone difference protein genes,to analyse sequence,The study further explore new train of thought for development and application of enzyme degradation.After the determination to the soil from each field by HPLC,this study find that the degrading strains FB5 has the ability of repair soil polluted by Fomesafen.The degradation rate by 61.19%after 7d.The strains can lead the Fomesafen half life cast down from 93d to 30d.We determined the PLFA of the soil using GC-MS,which has measure fungi/bacterial,GN/GP,stress indicator and the total PLFAs.The result shows that there are indifference between the experimental group and the control group and the degrading strains have a minor effect on the microfloral activity in soil.Based on Determination of fomesafen degrading bacteria in indoor degradation,we chose different time to do the mass spectrometry.The first degradation product(C14HgF3N2O6S)appeared at 24h with the nucleo-cytoplasm ratio of 388.8.The second degradation product(C14H11F3N2O5S)appeared at 48h with the nucleo-cytoplasm ratio of 375.6.To speculate the possible metabolic pathway.The basis of on the degradation bacteria residues with fomesafen.Extracted the difference of protein,finding 13 different protein spots,through the comparison of bioinformatics,get 13 point functions,suggesting that F3,F6 may be associated with herbicide metabolism,sequence analysis of gene cloning.Through information analysis,gene F3 sequences has the similarity homology with E.coli binding protein dps gene,is 100%;and the sequence gene F6 has the similarity homology Salmonella enteric subspecies salamae serovar Sophia bacteria S1635 outer membrane protein gene and E.coli SYW004 outer membrane protein A,it is 87%.The PCR products were sequence homology and phylogenetic tree,and speculated that the physicochemical properties of gene.