Fluorescence Sensing And Biological Application Of Zr(?) Nano-metal-organic Framework Materials

Phosphorus,as one of the important components of life,plays an important role in all aspects of life activities.Abnormal indicators of phosphate ions in the blood are closely related to diseases such as hyperparathyroidism,Frankenia syndrome,and hyperphosphatemia caused by chronic renal failure(CKD).The abnormal expression of alkaline phosphatase is an important indicator for the early detection of many diseases.Therefore,the development of analytical methods for the detection of phosphate and alkaline phosphatase is of great significance for the early diagnosis of major diseases such as renal failure.In view of the detection and treatment of hyperphosphatemia,a nanoprobe based on Zr(?)-MOFs was developed,which was used to detect hyperphosphatemia by using its fluorescence characteristics.Adsorption effectively reduces the level of blood phosphorus.Aiming at the detection of primary hyperparathyroidism and secondary hyperparathyroidism,this paper designed and prepared a simultaneous detection of phosphorus and alkaline phosphatase based on MOFs fluorescent nanoprobe.The diagnosis of both diseases is achieved by simultaneous detection of phosphorus and alkaline phosphatase.The details are as follows:1.Based on the specific action of zirconium(Zr(?))and phosphate,a metal-organic frame type fluorescent nanoprobe was developed.The material can detect phosphate in a highly sensitive and highly selective manner.Based on the specific adsorption between Zr(?)and phosphate,the blood phosphorus level is effectively reduced.At the same time,quantitative monitoring of blood phosphorus was achieved by using the fluorescence characteristics of Zr-MOFs.More importantly,Zr-MOFs have a better phosphorus lowering effect than commercially available phosphorus binders.Finally,the probe was applied to the hyperphosphatemia mouse model by means of gastric administration,and a good therapeutic effect was obtained.The use of MOFs to directly treat the phosphorus in the blood to treat hyperphosphatemia provides a new perspective for the future application of MOFs.2.Based on the specific coordination of phosphate groups with zirconium ions,combined with the specific recognition ability of nitrophenyl phosphate(PNPP)and alkaline phosphatase,a fluorescent nanoprobe based on P-MOFs was developed.The material not only quantitatively detects the alkaline phosphatase fluorescence by enzymatic catalyzing the fluorescent internal filtration effect of the substrate molecule,but also realizes the quantitative detection of phosphate fluorescence by using the specific action of zirconium ion and phosphate of MOFs material.Finally,we applied the material to the in vivo imaging study of a mouse model of chronic renal failure and a hypothyroidism-attenuated mouse model.The quantitative analysis of alkaline phosphatase and blood phosphorus levels enabled the differentiation of the two disease models.This study provides a powerful tool for early diagnosis of abnormalities in blood phosphorus and alkaline phosphatase induced by hyperparathyroidism.