| 1 BACKGROUND AND AIMSEpidemiology:Diabetes gastrointestinal diseases(diabetes gastroparesis,DGP)is a coumon complication of diabetes.According to statistics,up to 75%of diabetic patients have diabetic gastrointestinal disease,it shows significant gastrointestinal symptoms,those severe cases would show severe vomiting,diarrhea,lack of emaciation,difficult to control blood sugar.Modem medical treatment of diabetic gastrointestinal lesions is mainly based on the use of glycemic control and gastrointestinal motility promoting agents,which are symptomatic treatments and supportive therapies.Because the pathogenesis has not been elucidated,gastrointestinal mechanical force and motion study progress is lagging behind,the existing treatment could partially improve clinical symptoms,but the basic factors causing gastrointestinal disease still exist.There is high relapse rate after treatment,and gastrointestinal disorders can not be reversed radically,leading to further development of DGP.Worsening gastrointestinal motility disorders become the one of the outstanding problems of clinical reasons.Therefore,in-depth study of gastrointestinal lesions in diabetic mechanisms and seeking for effective control measures have become a major health problem.Gastrointestinal biomechanical remodeling in the pathogenesis of diabetic gastrointestinal lesions plays an important role:Biomechanics is an important part of biomedical engineering.Boreli firstly proposed that the gastrointestinal tract is the view of biomechanical organs,along with gastrointestinal development and application of motion detection technology.Researchers have begun to try to solve the meehanies of gastrointestinal motility problems.Scholars have basically reached consensus:Measuring gastrointestinal force or power should follow the principles of biomechanics.Biomechanical properties of the gastrointestinal tract,including the residual strain and stress distribution,and tissue elasticity,creep and viscosity,are depending on the structural characteristics of these characteristics gastrointestinal tissue,and can be measured by means of different biomechanical test.In diabetic state,gastrointestinal mucosa,muscle and submucosa collagen vary in degrees of hyperplasia,and submucosal layers of collagen cross-linking and organization of advanced glycation end protein deposition,resulting in gastrointestinal tissue structure and configuration changes,eausing morphological and biomechanical properties of the gastrointestinal tract remodeling.Further it affects gastrointestinal motility function in some way,which leads to diabetes gastrointestinal lesions.Thus,diabetes gastrointestinal biomechanical remodeling plays a role in causing gastrointestinal disorders.Protective effects of Tangweian on diabetic gastric gastrointestinal lesions:Previous clinical and basic studies have shown that Tangweian has improved vomiting,diarrhea and other gastrointestinal discomfort effect,achieved certain effects in the clinical treatment of diabetes and gastrointestinal disease.This study focuses on the biomechanics of the recovery of gastrointestinal pathway and its possible mechanisms to increase clinical basis,to enhance clinical efficacy.2 METHODS8 of 40 wistar male rats were randomly assigned to the normal control group,and the remaining were injected with 1%STZ.Diabetic rat model is considered established if the fasting plasma glucose ≥ 16.9 mmol/L three days after injection.successful modeling rats were divided into four groups,according to their weigh and fasting blood glucose:model group,high dose group,middle dose group and low dose group.Recorded weight every week and fasting blood glucose every four weeks.After 8 weeks,the rats were fasted overnight for 12 hours,put them under anesthesia,take the middle part of the jejunum 5cm in length,for the quantitative evaluation of gastrointestinal motility,passive expansion of the experimental analysis and zero-stress state.Experiments conducted in the organ bath,which filled with 95%oxygen and 5%carbon dioxide gas Krebs physiological solution(37oC).Test the pressurized gastrointestinal motility,gastrointestinal passive biomechanical properties,the zero-stress state of research,and through biomechanical analysis of data related to computer software.3 RESULTS(1)The study has found that blood glucose,glycated serum protein content,wall thickness,wall cross-sectional area,spread angle were different from normal group(P<0.05)8 weeks after STZ modeling;Tangweian has positive effect on glycated serum protein content,wall thickness and wall cross-sectional area,expand the eomer with improvement(P<0.05),and has dose-response relationship.(2)Compared with the normal group,model group rat intestinal AGE,RAGE expression was significantly strengthened(P<0.05);compared with the model group,treatment group with improved diabetic rat small intestine AGE,RAGE expression effect(P<0.05).(3)Linear regression analysis showed that advanced glycation end products and their receptors in the intestinal distribution and morphological and biomechanical parameters of relevance.4.CONCLUSIONSTangweian could improve STZ-induced diabetic rats morphological and biomechanical reconstruction,reducing the hardness of the intestinal wall,improve the expression of advanced glycation end products and their receptor in intestinal tissue layers.Linear regression analysis shows that patients with diabetes intestinal tissue remodeling and gastrointestinal abnormalities advanced glycation end products and their receptor expression correlated Tangweian may recover by improving advanced glycation end products and their receptors to restore DM rat gastrointestinal morphological shapes and biomechanical reconstruction,which is becoming one of the mechanisms that regulating gastrointestinal motility. |
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