Polymanamide Conductive Hydrogel Combined With Neural Tube To Repair Rat Sciatic Nerve Defect

Objective:To study the preparation and properties of gelatin-graft-polyaniline(GP)hydrogel.Methods:GP was synthesized by means of direct doping technique and camphorsulfonic acid was used as dopant.The copolymer was dissolved in water and then cross-linked by EDC.The morphology and chemical feature,swelling ratios,dissolubility,biocompatibility and conductivity were observed.Results:GP hydrogels had good conductivities(10-4-10-3S/cm).The electrical property was enhanced with the content of polyaniline.Nonetheless,when the amount of polyaniline improved to a certain degree,the solubility was greatly reduced and swelling ratio increased at the same time.Conclusion:GP hydrogel is a biodegradable conductive material and has great application prospect in tissue engineering.Objective:To evaluate the efficacy of regeneration in rat sciatic nerve defect repaired by collagen neural guided tube combined with gelatin-graft-polyaniline hydrogel(GPH)and polycaprolactone-gelatin(PCL/G)electrospinning filmMethods:We fabricated polycaprolactone-gelatin electrospinning film and collagen film to manufacture a double layer tube,GPH as lumen filler.Bridging a 10mm long rat sciatic nerve defect by GPH-PCL/G-collagen(GPH group)nerve guided tube,autograft(AG)and no hydrogel(PCL/G)group as control group.A combination of electrophysiological assessment and transmission electron microscopy(TEM)observations for all regenerated nerves were conducted to study axonal regeneration and functional recovery.Results:The nerve was regenerated in all groups in this study,a combination of electrophysiological assessment and histomorphometry analysis of regenerated nerves showed that axonal regeneration and functional recovery in GPH group were similar to AG group(P>0.05).In AG and GPH groups,most myelinated nerves were matured and densely arranged with a uniform structure.Conclusion:GPH hydrogel has a significant effect on the improvement of sciatic nerve regeneration in rats,which can be used as a new alternative for histochemical artificial neural material.