Bushen Yanggu Decoction Induces Apoptosis Of Drug-resistant Multiple Myeloma Cell Lines

Objective:To study the effects and mechanisms of BuShenYangGuTang on proliferation and apoptosis of multiple myeloma cell line KM3/BTZ.Methods:The inhibitory effect of BuShenYangGuTang on cell proliferation was assessed by CCK-8.BuShenYangGuTang induced KM3/BTZ cell cycle arrest was analyzed by flow cytometry after propidium iodide staining.Flow cytometry was also used to analyze the cell apoptosis after Annexin V-FITC staining.The expressions of Wnt,β-catenin and DDK1 were determined by RT-qPCR and Westernblot assay.Results:BuShenYangGuTang inhibited the KM3/BTZ cell proliferation in a dose-dependent manner.The expression levels of Wnt and β-catenin were decreased,the expression level of DKK1 was increased,and the cell cycle was arrested in G0/G1 phase after treatment with BuShenYangGuTang.Conclusion:BuShenYangGuTang could inhibit the proliferation,arrest cell cycle and induce the apoptosis in KM3/BTZ cells,which may be related to the abnormal expressions of nt,β-catenin and DDK1.