A Preliminary Study On The Anti-inflammatory Bone Destruction Of Miao Medicine Sargentodoxa Cuneata And Its Mechanism

Miao medicine Sargentodoxacuneata as a traditional Chinese medicine,it is commonly used in clinical practice to promote blood circulation and relieve pain.The more complicated pathological process of inflammatory bone destruction makes it a hot spot in current research.In this study,Sargentodoxacuneata was studied as the object of study to explore its activity in the destruction of the bone of inflammation,to find effective compounds against inflammatory bone destruction,and to conduct a preliminary study on its mechanism.In this study,Miao medicine Sargentodoxacuneata was selected as the research object.The RAW264.7 cell line was induced by lipopolysaccharide(LPS).The inflammatory cell model was established.The model and IL-6 and TNF-α release were used as indicators.The results showed that the concentration of NO,IL-6 and TNF-α in the ethyl acetate layer was significantly different from that of the negative group(p<0.01),and it was concentration-dependent.The inflammatory osteoclast model was established using induction factors,and the model was used to determine the number of Trap positive cells,the activity of Trap enzyme,the lacunae area of the bones,and the release of NO,IL-6,and TNF-α as indicators.The ethyl acetate layer has a good anti-inflammatory bone destruction activity.Using epicatechin,hydroxytyrosol and vanillin-ethyl ketone as research objects,the inflammation model was used to find that each concentration group had anti-inflammatory activity,and the combined release of NO,IL-6 and TNF-a resulted in the discovery of catechins.The low concentrations of hydroxytyrosol and vanilloidone all had better anti-inflammatory effects.The inflammatory bone destruction model was used to determine the number of Trap positive cells,the activity of Trap enzyme,the area of bone lacunae,and the release of NO,IL-6 and TNF-α.The results showed that hydroxytyrosol and catechin have better Anti-inflammatory bone destruction activity.The mechanism of the anti-inflammatory bone destruction activity of catechins and hydroxytyrosol was preliminary studied.The total mRNA and protein of the samples at different time were obtained by using the anti-inflammatory bone destruction model,and reverse transcription and quantitative PCR analysis were performed.The expression of TRAF6,NFATc1,IL-6 and TNF-α genes,and the expression of TRAF6 and NFATc1 protein by Western blotting,it was found that over time,the drug group inhibited the expression of genes and proteins at 12 hours.It is optimal,but as the drug concentration increases,its expression also decreases.All polar parts of Sargentodoxacuneata showed significant anti-inflammatory and anti-inflammatory bone destruction activity.Epicatechin,hydroxytyrosol and vanilloidone had significant anti-inflammatory effects but had significant anti-inflammatory bone destruction activity.Can be used as a clinical potential drug for further research.