| Purpose:To observe the effect of Pi-danning on blood sugar and body weight,and the effects of NF-κ B signal and TNF-α transcription in rats with impaired glucose regulation,and to further explore the mechanism of Pi-danning in the treatment of splenic trichomycosis.Material and method:SPF grade Wistar rats,male,60-70 days old,body weight 220 ±20 g.One week later,100 male rats were randomly divided into two groups: blank group(n = 20)and model group(n =80).Then the rats in the model group were induced by high-glucose and high-fat diet to establish the model of impaired glucose regulation.The rats in the model group were fed with high-glucose and high-fat diet,and the rats in the model group were fed with high-glucose and high-fat diet.The rats in the model group were fed with high-glucose and high-fat diet.Rats in blank control group were fed with routine diet for 12 weeks.After the successful evaluation of the model,the successful rats were randomly divided into three groups: model control group,spleen catenin group and metformin control group.Corresponding interventions were given: The rats in the blank control group and the model control group were perfused with distilled water of 5ml kg-1 ·d-1 through the mouth,and the rats in the spleen channin group were given orally with 10 g kg-1 ·d-1 spleen catenin decoction.Metformin control rats were given 150 mg kg-1 ·d-1 metformin suspension orally.The intervention in each group was once a day for 4 weeks.One hour after the last administration,the rats in each group were weighed and recorded.Blood samples were collected from the tip of the tail and blood glucose was measured by a rapid blood glucose meter.Then the rats were killed by excessive anaesthesia and placed on the ice,the lower abdomen was cut open,the fat tissue around the epididymis was collected and placed in the 2ml cryopreservation tube,and stored in the refrigerator at-70 ℃ for reserve.Six samples were randomly selected to detect the expression level of TNF-α and NF-κ B m RNA in adipose tissue by RT-PCR method,and6 samples were randomly selected to detect the expression level of TNF-α and NF-κ B in adipose tissue by western-blot method.Results:1.After modeling,the FBG and 2h PG of the blank control rats were 4.54 mmol/L and 6.73mmol/L respectively.Therefore,FBG + 1.96 S was 6.5 mmol/L in blank rats and 8.69 mmol/L in 2h PG + 1.96 S rats.The results of FBG and 2h PG measured in each group showed that FBG was higher than 6.5 mmol/L,lower than 7.0 mmol/L;OGTT was higher than 8.69mmol/L,lower than 11.1 mmol/L;and random blood sugar was lower than 16.7 mmol/L in 43 rats.2.The results of weight record of each group showed that there was no significant difference in the weight of each group before modeling,and there was no significant difference in the weight of each group before the establishment of the model.After the establishment of the model,the weight of the rats in each group was significantly different before administration.Before administration,the weight of the rats in the model control group,the spleen catenin group and the metformin control group was significantly higher than that in the blank control group(P < 0.01),while the model control group and the spleen chensin group were significantly increased(P < 0.01).There was no significant difference in body weight among the three groups of metformin control group(P > 0.05).After administration,the weight of the rats in the model control group,spleen heat group and metformin control group were significantly higher than those in the blank control group(P < 0.01).However,compared with the model control group,the weight of the rats in the spleen-catenin group and metformin control group were significantly lower than those in the model control group(P < 0.01).However,there was no significant difference in body weight between the two groups(P >0.05).3.The results of blood glucose detection before and after administration showed that fasting blood glucose and OGTT2 h blood glucose were significantly higher in model control group,spleen omangning group and metformin control group than those in blank control group before and after administration(P < 0.01),and there was significant difference between the two groups(P < 0.01).However,there was no significant difference in fasting blood glucose and OGTT2 h blood glucose among the three groups: model control group,spleen-catenin group and metformin control group(P > 0.05).After administration,compared with the odel control group,the fasting blood glucose of rats in the spleen-catenin group and metformin group decreased significantly(P < 0.05 or 0.01),but the fasting blood glucose in the spleen-catenin group was higher than that in the normal control group(P < 0.01).There was no significant difference in fasting blood glucose between metformin control group and blank control group(P > 0.05).4.The expression levels of TNF-α,NF-kappa B m RNA and protein in adipose tissue of rats in each group were detected.The results showed that compared with the blank control group,the model control group,the spleen catenin group and metformin control group,the expression of TNF-α in adipose tissue of rats was significantly higher than that of the control group.The expression of NF-κ B m RNA was up-regulated significantly(P < 0.01).Compared with the model control group,the expression levels of TNF-α and NF-κ B m RNA in adipose tissue of rats in spleen omitting group and metformin control group were significantly lower than those in model control group(P < 0 01),and there was significant difference between the two groups(P < 0 01).There was no significant difference in the expression of TNF-α and NF-kappa B m RNA in adipose tissue between the two groups(P >0 05).Conclusion:1.A stable glucose-regulated rat model was established by continuous feeding with high-glucose and high-fat diet.2."Spleen governs transportation and transformation" theory is used to treat splenic macula.Based on this theory,Pi-danning can effectively inhibit the expression of TNF-alpha and improve insulin resistance.3.Pyrenin is similar to metformin in regulating body weight(fat metabolism),blood sugar,and 2 hours postprandial blood sugar.Inhibition of over activation of NF-κ B signaling pathway may be one of its mechanisms. |
PDF Full Text Request