| Purpose:To study the effect of Chinese herbal compound Pidan Ning on insulin resistance and protein kinase B tyrosine phosphorylation in skeletal muscle cells of diabetic rats.Materials and methods: SPF grade Wistar rats,male,60-70 days old,body weight 220 ±20 g.One week later,100 male rats were randomly divided into two groups: blank group(n = 20)and model group(n = 80).Then the rats in the model group were induced by high-glucose and high-fat diet to establish the model of impaired glucose regulation.The rats in the model group were feded by high-glucose and high-fat diet,the rats were free to drink water,and the room temperature was controlled at 18-20 ° C,and the relative humidity was 30%-70%.Feeding time lasted for 12 weeks.Rats in blank control group were feded with routine diet for 12 weeks.After 12 weeks of routine diet and high-glucose and high-fat diet,the rats in the model group were carried out about the oral glucose tolerance test(OGTT)to determine the successful of the model of impaired glucose regulation.the successful rats were randomly divided into three groups: model control group,spleen catenin group and metformin control group.Corresponding interventions were given: The rats in the blank control group and the model control group were perfused with distilled water of 5ml kg-1·d-1 through the mouth,and the rats in the spleen channin group were given orally with 10 g kg-1·d-1 spleen catenin decoction.Metformin control rats were given 150 mg kg-1·d-1 metformin suspension orally.The intervention in each group was once a day for 4 weeks.Testing the rats of each group no matter before or after given the treatment around their weight,fasting blood glucose(FBG)and fasting insulin(FINS),and insulin resistance was evaluated by high insulin-positive glucose clamp technique.At the end of the experiment,all rats were sacrificed and the correlation between the degree of PKB tyrosine phosphorylation and insulin resistance was analyzed.Results: 1.Evaluation of fasting insulin and glucose infusion rates: Before the intervention of the two drugs,the fasting insulin of the model control group,the Pidan Ning treatment group and the metformin control group were significantly higher than the normal control group(P<0.01),and the GIR of the three groups were significantly lower than the normal control group(P< 0.01),and there was no significant difference in fasting insulin and glucose infusion rate between the three groups(P>0.05).After drug intervention,fasting insulin was significantly higher in the Pidan Ning treatment group and metformin group than in the normal control group(P<0.01).However,they were significantly lower than the model control group(P<0.01).The GIR between the two groups was significantly lower than that of the normal control group(P<0.01),but they were significantly higher than the model control group(P<0.01).There was no significant difference between fasting insulin and GIR(P>0.05).2.Evaluation of the degree of tyrosine phosphorylation of protein kinase B in skeletal muscle cells: Before the two drugs were intervened,the degree of PKB tyrosine phosphorylation in skeletal muscle cells of model control group,Pidan Ning treatment group and metformin treatment group was significantly lower than that of normal control group(P<0.01),and there was no significant difference in the degree of PKB tyrosine phosphorylation in the skeletal muscle cells of the above three groups(P>0.05).After treatment with Pidan Ning and metformin,the degree of PKB tyrosine phosphorylation in skeletal muscle cells of Pidan Ning treatment group and metformin group was lower than that of normal control group(P<0.01).but were significantly higher than the model control group(P<0.01),and there was no significant difference between Pidan Ning treatment group and the metformin treatment group(P>0.05).3.The correlation between insulin resistance and the degree of protein kinase B tyrosine phosphorylation in skeletal muscle cells.The degree of tyrosine phosphorylation of protein kinase B in skeletal muscle was significantly positively correlated with glucose infusion rate(r = 0.820,P < 0.01).Conclusion: 1.The Pidan Ning can obviously improve the insulin resistance of the rats with impaired glucose regulation.2.Pidan Ning can significantly increase the degree of receptor substrate 1 tyrosine phosphorylation in skeletal muscle cells of rats with impaired glucose regulation.3.Pidan Ning can improve insulin resistance in rats with impaired glucose regulation by increasing the regulation of IRS-1 tyrosine phosphorylation in skeletal muscle cells of rats with impaired glucose regulation. |
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