| Objective: Intervene experimental autoimmune uveitis(EAU)rats with Qufeng Huoxue Pills to observe the levels of interleukin-2(IL-2)and forkhead box protein 3(Foxp3)in rat serum influences.Methods: Using random number table method,12(48 eyes)male Lewis rats were randomly selected and divided into blank groups.The remaining 36 rats were injected with 100μ L of vitamin A binding protein containing 35μg interretinal photoreceptors(inter-Photoreceptor retinoid binding protein,IRBP and 35μg Freund’s adjuvant(CFA)chyle,actively immunize Lewis rats,and establish an EAU rat model.After successful modeling,rats are treated under a slit lamp microscope every day The anterior segment was observed,and according to its inflammation,Caspi score was used to score the inflammation of the anterior segment of rats.On the 7th day after immunization,one was selected from the blank group and the other was selected from 36 EAU rat models.The eyeballs were removed and pathological sections were made,stained by HE,and then histopathologically examined.After successful modeling,33 EAU rats were randomly divided into three groups according to the random number table method: model group,Qufeng Huoxue Pill low-dose group and Qufenghuoxue pill high-dose group.For 14 consecutive days,all rats were administered by intragastric administration,using ELISA method to detect the relative table of IL-2 and Foxp3 in serum of EAU rats the amount.Results: 1.Verification of the EAU rat model: On the third day after the model was established,a slight inflammation reaction was observed under the slit lamp microscope,which showed that the blood vessels on the iris surface were slightly dilated,the limbal blood vessels were congested,and some pupils were diminished;069±0.25).On day 5,corneal edema,pupil dilation in some rats,iris congestion and edema,unclear texture,varyingdegrees of anterior chamber opacity,posterior corneal wall deposits,aqueous humor shining,weakened red light reflection in the fundus;ocular inflammation score(1.94±0.23).On the 7th day,there was obvious inflammation in the anterior segment of the eye,severe turbidity in the anterior chamber,turbidity of the aqueous humor,pupillary membrane closure,and grayish white flocs on the pupillary margin,showing a significant uveitis reaction;eye inflammation score(2.97±0.29).On the 7th day after immunization,pathological sections of the rat’s eyeball showed that there were varying degrees of inflammatory cell infiltration in the subcorneal,iris root,around the ciliary body,retina and other parts of the rat.The above indicates that the EAU rat model was successfully established.2.ELISA test results showed that: IL-2 and Foxp3 at each time point in the model group were significantly higher than that in the blank group,and the difference was statistically significant(P<0.01).The expression of IL-2: Compared with the model group,the expression of Qufeng huoxue Pill low-dose group was decreased,and the expression of Qufeng huoxue Pill high-dose group was significantly reduced,the difference was statistically significant(P<0.01).Foxp3 expression:Compared with the model group,the expression of Qufenghuoxue Pill low-dose group decreased,the difference was statistically significant(P<0.05);Qufeng huoxue Pill high-dose group decreased,the difference was statistically significant(P<0.01).Conclusion: 1.Qufeng Huoxue Pill can reduce the concentration of IL-2and Foxp3 in the serum of EAU rats,indicating that the drug can inhibit the production of IL-2 and Foxp3 and interfere with the expression of both,thereby achieving the purpose of treating uveitis.2.Qufeng Huoxue Pill may disrupt the balance of IL-2 and Foxp3,so that Foxp3 promotes the restriction of IL-2 expression,thereby achieving anti-inflammatory effect. |
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