Study On The Mechanism Of Bushen Yisui Capsule And Its Monomer Catalpol In Promoting The Proliferation, Differentiation And Migration Of OPC

Study on the Mechanism of Bushen Yisui Capsules in Promoting OPC Proliferation,Differentiation and MigrationObjective:Multiple sclerosis(MS)is an autoimmune-mediated inflammatory degenerative disease of the central nervous system(CNS).Its multiple lesions lead to a variety of clinical symptoms,which can cause movement and feeling.Visual and other functional abnormalities,often showing the course of relapse and remission.Repeated episodes of the disease lead to the accumulation of nervous system damage,which seriously reduces the patient’s life and even quality of life,and has a high recurrence rate and disability rate.Based on the previous stage,this research studies the Bushen Yisui Capsule and Catalpol promote the expression of the transcription factors Oligl/2,myelin basic protein(MBP),and oligodendrocyte precursor The mechanism of cell(oligodendrocyte progenitor cells,OPC)proliferation,differentiation and migration provides a scientific basis for Bushen Yisui capsule and its monomer catalpol to treat MS/experimental autoimmune encephalomyelitis(EAE).Methods:The mice in each group of EAE began to develop on the eighth day,and the incidence rate was 100%.There was no significant difference in the onset latency of each group.The modeling groups reached the peak within 15-16 days.Electron microscopy showed that the myelin injury in the PA group,catalpol group,and Bushen Yisui capsule group were repaired to varying degrees.Immunofluorescence and results showed that compared with the model group,after drug intervention,the expression of NEUN and MBP in the catalpol group and the Bushen Yisui capsule increased in the brain,and the expression of JNK decreased;NSC can be differentiated into OPC under certain conditions.Compared with the cells in the blank control group(Normal control,NC),the absorbance of the model group(Model,MO)cells CCK-8 was significantly reduced,the cell protrusion rate was significantly reduced,and the Oligl integrated optical density was significantly reduced.Compared with the MO group,catalpol at different concentrations can significantly increase the survival rate of OPC cells,protect OPC cell processes,and increase the expression of Oligl protein Among them,catalpol at a concentration of 10 μM/L has the best effectResults:The mice in each group of EAE started to develop disease on the eighth day,and the incidence was 100%.There was no significant difference in the incubation period in each group.The modeling groups reached a peak at 15-16 days.HE staining showed that the EAE model groups showed cerebral inflammatory infiltration and neural stem cell nuclei shrinking on the 18th day,and a large number of lymphocytes gathered around the blood vessels Alleviated.Electron microscopy showed that myelin sheath damage in PA group,catalpol group,and Bushen Yishui capsule group were repaired to varying degrees.Immunofluorescence and immunohistochemical results showed that after drug intervention,the expressions of Oligl/2 and MBP in the brain of catalol group and Bushen Yisui capsule increased.Under certain conditions,NSC can be differentiated into OPC.Compared with the normal control(NC)cells,the absorbance of CCK-8 in the model group(MO)cells is significantly reduced,the rate of cell protrusion is significantly reduced,and the integral optical density of Olig1 is significant.reduce.Compared with the MO group,catalpol at different concentrations could significantly increase the survival rate of OPC cells,protect the OPC cell protrusions,and increase the expression of Oligl protein.Among them,catalpol had the best effect at a concentration of 10 μM/L.Conclusion:Catalpol protects OPC after AAPH injury and can obviously promote the proliferation of OPC cells.Bushen Yisui Capsule and Catalpol have neuroprotective effect on EAE mice and can promote the regeneration of myelin sheath.The mechanism may be that Catalpol and Bushen Yisui Capsule can promote the expression of Treg by reducing the expression of c-Jun N-terminal kinase(JNK)and increasing the expression of Neuronal nuclei(NEUN)and MBP NOV secretion enhances OPC proliferation and promotes its differentiation into OL.Catalpol and Bushen Yisui Capsules can improve the clinical symptoms of EAE mice,reduce the neurological score,promote the proliferation and differentiation of OPC,and thus promote the regeneration of myelin.To clarify the partial mechanism of Bushen Yisui Capsule in the treatment of demyelination.