The Characteristics Of Free Radical Content In Synovial Fluid Of Patients With Knee Osteoarthritis And Its Correlation With Syndromes

Objective:1.Discover the relationship between MDA,SOD and different syndromes in KOA(knee osteoarthritis,KOA)patients via the observation of concentration of MDA and SOD with different syndromes,providing material basis for the objectification of the TCM(Traditional Chinese Medicine)syndrome.2.Discover the correlation between the concentration of MDA,SOD and the scores of TCM syndrome,namely the seriousness of the diseases,through comparison the MDA,SOD concentration of the KOA patient with same syndromes,providing objective evidences for the standardization of TCM syndrome and clinical precise dialectical treatment.Methods:The research involves 54 KOA patients in our hospital from January 2019 to May 2019,including 15 males and 39 males,with the ages ranging from 27 to 77,62.54±12.02 years old on average.All the patients are divided into,based on the TCM identification standards,phlegm stasis syndrome group,which contains 23 cases and liver-kidney deficiency syndrome group which contains 31 cases.The MDA and SOD concentration are measured by thiobarbital method and xanthine oxidation method respectively after harvesting the synovial fluid from the patients and then adopt the TCM syndrome standard to evaluate the seriousness of the syndromes.Results:1.Average MDA concentration in synovial fluid of the patients phlegm stasis syndrome group is(2.10±1.67),while that in the patient of liver-kidney deficiency syndrome group is(1.40± 1.32).It thus indicate that the MDA concentration in phlegm stasis patients is higher than that in liver-kidney deficiency patients,which is also statistically significant(P<0.05);2.Average SOD concentration in synovial fluid of the patients of phlegm stasis syndrome group is(55.01±12.30),while that in the patients with liver-kidney deficiency syndrome is(47.93±14.22),which is lower than that in phlegm stasis patients and it is also statistically significant(P<0.05);3.By statistical study of the concentration of MDA,SOD concentration in the synovial fluid in the patients with phlegm stasis syndrome,we found that in these patients,the TCM syndromes scores are positive correlated with the synovial fluid MDA concentration and it is statistically significant(r=0.532,P<0.05=while the synovial fluid SOD concentration is also positively correlated but it is not significant(r=0.172,P>0.05);4.By statistical study of the concentration of MDA,SOD concentration in the synovial fluid in the patients with live-kidney deficiency syndrome,we found that in these patients,the TCM syndromes scores are positive correlated with the synovial fluid MDA concentration but is statistically non-significant(r=0.088,P>0.05),while the synovial fluid SOD concentration is positively correlated and is statistically significant(r=0.424,P<0.05).Conclusions:1.Different concentration of synovial fluid MDA,SOD among different KOA patients manifested the differences in the syndromes.2.Synovial fluid MDA and SOD concentrations of KOA patient are correlated with the TCM syndrome scores,representing the seriousness of the syndromes.3.The two features described above are in consistent with etiological and pathological analysis by TCM under different syndromes in this diseases.This provides the material basis for the syndrome objectification,providing evidence for the syndrome standardization and precise dialectical treatment,enriching the point of syndromes identification.