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A new method for single cell gene expression analysis by cDNA synthesis, barcoding and sequencing

Posted on:2011-12-11Degree:M.SType:Thesis
University:Adelphi UniversityCandidate:Katz, JustinFull Text:PDF
GTID:2444390002453647Subject:Biology
Abstract/Summary:
A new method for single cell genetic expression analysis has been proposed. The goal is to sequence the cDNA from a large population of single cells in such a way that each strand of sequenced cDNA can be distinguished from the others, both in transcript and by which cell they each came from. Three different approaches are proposed and two have been tested. This project is the progression of the methodology, with each step discussed in detail, as well as newly proposed methods.
Keywords/Search Tags:Single, Cell, Cdna, Proposed
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