The biodecoloration of textile dyes by the white rot fungus Trametes versicolor

Six species of white rot fungi (Bjerkandera sp. BOS55, Lentinus edodes, Phanerochaete chrysosporium, Pleurotus ostreatus, Trametes hirsuta, Trametes versicolor) were screened for their ability to decolorize 6 dyes on solid culture medium. Of these, Bjerkandera sp. BOS55 and T. versicolor ATCC 20869 decolorized the greatest number of dyes to the greatest extent and were chosen for aqueous decoloration studies. P. chrysosporium was included for comparison purposes.; The aqueous decoloration of 7 structurally diverse dyes was examined in static and agitated cultures. Decoloration required culture agitation (200 rpm), which resulted in the formation of mycelial pellets.; Batch cultures of T. versicolor completely decolorized all the dyes tested at concentrations from 20 to 60 ppm, either individually, in mixtures or sequentially. The culture medium was completely decolorized, with no visible sorption of the dye to the mycelia. The choice of buffer was critical, with 2,2{dollar}spprime{dollar}-dimethylsuccinic acid providing good pH control during decoloration. Bjerkandera sp. BOS55 and P. chrysosporium had limited ability for sequential decoloration and were not further studied. (Abstract shortened by UMI.)...