| Capillary electrochromatography (CEC) is an important analytical tool that is widely used in chemical separations. A new open tubular approach to CEC was investigated which overcomes most of the problems encountered in the packed capillary format. In this new format, the inner walls of capillaries are etched in order to increase the surface area and then an organic moiety is attached to the etched surface by a silanization-hydrosilation reaction scheme. The organic moiety used in this research was 1-octadecene (C-18) and the performance of this type of capillaries was compared to that of the bare capillaries.; Various mixtures including drugs and proteins were separated using three different types of capillaries. The effects of several parameters on the performance of these capillaries was also studied. Moreover, electroosmotic flows in different types of capillaries were measured and the effect of experimental variables like buffer pH and voltage were investigated. |
