ENZYMATIC PEPTIDE SYNTHESIS USING AN IMMOBILIZED CARBOXYPEPTIDASE Y REACTOR - DISPLACEMENT CHROMATOGRAPH SYSTEM

Immobilized carboxypeptidase Y was employed for enzymatic peptide synthesis. The enzyme was immobilized by glutaraldehyde coupling onto microparticulate amino-silica and was used in packed bed reactors in a recirculating mode. Mercuric derivatives of the enzyme were examined for their efficacy in catalyzing peptide synthesis reactions using amino acid methyl esters as the amine component. The kinetics of the Bz-Arg-Met-NH...