Preliminary Study On The Regulation Of N~6-methyladenosine Nucleic Acid Modification By Light

Epigenetics is an important part of genetics.In recent years,nucleic acid modification has become a hot topic in the field of epigenetics.At present,nucleic acid modification is widespread in plants,yeast and viruses.nucleic acid modification has important biological functions in plants.However,in plants,how external signals regulate nucleic acid modification is still unclear,and light is one of the most important external environmental signals for plants.So,we want to see if there was a relationship between light and modification of 6-methyladenine nucleic acid.This paper conducted a preliminary study on light and the modification of 6-methyladenine nucleic acid.Following results were obtained:1?The overall m~6A modification level in wild type(Columbia-0,Col-0)with or without white light conditions was detected respectively.The results showed that the m~6A modification level detected in the wild type under white light were significantly higher than detected in the wild type under dark light.indicating that light could induce m~6A modification.2?The overall m~6A modification levels in wild type and corresponding mutants to blue light,red light and far-red light conditions was detected respectively.The results showed that only the blue light receptor mutant cry1 cry2 showed significant differences of m~6A level detected in the wild type under blue light treatment,indicating blue light induced m~6A modification.3?The phenotypic observation of wild-type and mutant fip37-4 hypocotyl under different monochromic light in Arabidopsis thaliana.The results showed that blue light had no obvious inhibition effect on mutant fip37-4 hypocotyl elongation.4?Yeast double hybrid vectors MTA-p GADT7,MTB-p GADT7,FIP37-p GADT7and ALKBH10B-p GADT7 were successfully constructed.Through the yeast two-hybrid experiment,the results showed that the interaction between the blue light receptor CRY1and FIP37 was found in blue light.5?Atdamt-1 mutant plants were successfully identified,and 6m A level in the wild type and Atdamt-1 mutant were detected.The results showed that 6m A levels in the wild type were significantly higher than Atdamt-1 mutant plants.6?At DAMT-1 protein was successfully induced and purified.These preliminary results provided clues for studies on functional relationship between the light receptor and the modification of 6-methyladenine nucleic acid in Arabidopsis thaliana.We will continue our study on molecular mechanism of light signal and the modification of 6-methyladenine nucleic acid.