Differentiation Function Of Bone Marrow Mesenchymal Stem Cells And Differential Gene Expression Profile

Objective: To investigate the effect of steroid coenzyme A desaturase1(SCD1)overexpression on osteogenic differentiation of bone marrow mesenchymal stem cells(BM-MSCs),and to analyze the changes of whole genome expression profile by gene chip technology.Methods: BM-MSCs were transfected with SCD1 lentivirus.The overexpression and activity of SCD1 in BM-MSCs were detected by RT-PCR and C14 technique.OC and other related osteogenesis indexes were detected by Western blot and alizarin red staining after BM-MSCs were induced and cultured.The effect of overexpression of SCD1 on osteogenic differentiation expression profile of BM-MSCs was further examined by whole gene chip.Results: SCD1 was successfully overexpressed in BM-MSCs,and SCD1 activity in overexpressing group was significantly higher than that in control group.The levels of alkaline phosphatase(APL)and osteocalcin(OC)in the overexpression group were higher than those in the control group at 7 and14 days after osteogenesis induction(p < 0.05).Alkaline phosphatase staining and alizarin red staining were more in the overexpression group than in the control group at 7 and 14 days after osteogenesis induction.The results of gene expression microarray showed that 2896 differentially expressed genes were detected by overexpression of SCD1 in bone marrow mesenchymal stem cells.Gene pathway analysis showed that the interferon pathway was the most significant pathway(p<0.05).Conclusion:Overexpression of SCD1 can promote the osteogenic differentiation of BM-MSCs,which may affect the osteogenic differentiation function through interferon pathway.This finding may provide important thinking and potential therapeutic strategies for fracture healing,and is worthy of further study.