Phylogenetic And Taxonomical Study On Pterygiella(Orobanchaceae),A Genus Endemic To Southwest China,and Its Related Taxa

Pterygiella is endemic to Southwest China,although some classified evidence of the genus accumulated,there are many problems in classification of the genus and its related taxa,so it's necessary to clarify classification disputes.In this study,Pterygiella and its related taxa were selected as the research object.Based on 375 new sequences in 48 taxa(48 ITS sequences,47 rbcL sequences,47 rpl16 sequences,48 rps16 sequences,48 rps2 sequences,48 trnH-psbA sequences,46 trnK-matK sequences,43 trnS-G sequences)and 89 existing sequences in 24 taxa downloaded from NCBI(24 ITS sequences,19 rbcL sequences,13 rps16 sequences,14 rps2 sequences,18 trnH-psbA sequences,1 trnS-G sequence),maximum likelihood(ML)and Bayesian inference(BI)analyses were used to reconstruct phylogenetic trees.Based on the field observation and indoor analysis,the growth environment,life form and morphological characters of the Pterygiella and Phtheirospermum tenuisectum complex in the field were studied.After statistical analysis of the specimen materials,color photographs accumulated in recent years,and the geographic collection information recorded of specimens from the Internet,the geographical distribution pattern of Pterygiella was mainly studied.The seed micromorphology of Pterygiella,Phtheirospermum japonicum and 2 species of Phtheirospermum tenuisectum complex was studied.The main conclusions of this study are as follows:1.The molecular systematics and morphological-geography study of Pterygiella and its related taxa,shows:Pterygiella was well supported as monophyletic in both ITS and ITS+cpDNA analyses,Phtheirospermum tenuisectum complex was well supported as monophyletic in ITS+cpDNA,Phtheirospermum parishii and Pseudobartsia glandulosa together form monophyletic in both ITS and ITS+cpDNA analyses,Phtheirospermum japonicum was well supported as monophyletic in both ITS and ITS+cpDNA analyses,and there are obvious morphological differences and independent geographical distribution between these 4 clades.Therefore,this study does not support the predecessors to merge Pterygiella,Phtheirospermum tenuisectum complex and Phtheirospermum parishii into a genus,but supports Pterygiella and Phtheirospermum tenuisectum complex as an independent genus.At the same time,admitted Pseudobartsia and Phtheiroumpermum as an independent genus.2.Through morphology-geography and seed micromorpholog study of Pterygiella,found that: In the classification of Pterygiella,indumentum,branching conditions,and the ratio of length to width of leaves have important guiding significance.Racteoles and corollas are unstable and vary depending on the type and environment,and need to be treated differently.Each species has its own geographical distribution pattern.Seed micromorphology has significant differences among species,and has significant classification value in the definition of species.3.Pterygiella is composed of six species,including Pterygiella nigrescens Oliv.,Pterygiella duclouxii Franch.,and Pterygiella cylindrica Tsoong,as well as three new species discovered in this study,Pterygiella luzhijiangensis Huan C.Wang,Pterygiella trichosepala Huan C.Wang & M.Y.Yin,and Pterygiella kunmingensis Huan C.Wang & M.Y.Yin.The division of these species has significant support in molecular systematics,morpho-geography and seed micromorphology.4.This study established a new genus,Pseudopterygiella,including four species,namely Pseudopterygiella tenuisectum(Bur.et Franch.)Huan C.Wang & M.Y.Yin and Pseudopterygiella muliense(C.Y.Wu & D.D.Tao)Huan C.Wang & M.Y.Yin,and two new species discovered in this study,Pseudopterygiella huizensis Huan C.Wang & M.Y.Yin.and Pseudopterygiella jinshajiangensis Huan C.Wang & M.Y.Yin.5.Both molecular systematics and morphological-geographic evidence supported move Phtheirospermum parishii to Pseudobartsia.The new combination is named: Pseudobartsia parishii(Hook.F.)Huan C.Wang & M.Y.Yin.