DNA Molecular Lock Based On Nicking Enzyme

In recent years,with the continuous development of Internet technology,the era of artificial intelligence and big data have also followed.The progress of science and technology not only provides people with unprecedented convenience,but also comes with the issue of information security.Under the background of the rapid growth of information technology,information security is a huge challenge facing the continuous progress of today's society,and how to realize information security protection has become a hot topic of current people's attention.At the same time,the rapid development of nanotechnology exhibits excellent characteristics and provides powerful tools for the construction of new nanomolecular devices.It has become an inevitable trend to use this strong nanotechnology to develop a new molecular information security device.Among the many nanomanipulation technologies,the Nicking enzyme as a special restriction endonuclease has a unique nicking ability.This enzyme can specifically recognize the recognition domain in the DNA double strand and nick the specific site,thereby inhibiting or promoting the reaction.The unique cutting characteristics of the Nicking enzyme provide a powerful tool for the construction of this DNA molecular lock.Therefore,in this paper,further research is carried out on the construction of the logic operation module and the molecular lock based on the Nicking enzyme.The specific work is as follows:First,logical operation module based on Nicking enzyme and its hierarchical control.In the design of the logic operation module,the cutting characteristics of the Nicking enzyme is explored,and three YES-YES cascade circuits are designed and implemented.Subsequently,the cascade characteristics of the substrate nick by the Nicking enzyme is explored,and the YES-AND cascade circuit based on the Nicking enzyme is designed and implemented.The construction of the cascade circuit can realize hierarchical manipulation under the control of the Nicking enzyme,thereby performing normal logic operations.Second,cooperative control of inhibition module and realization of molecular lock.In order to construct a high-security DNA molecular lock,in this study,a series of Inhibit logic circuits is constructed to explore the cooperative manipulation of inhibitor modules by enzymes with different input combinations.Through the construction of the logic circuit,it is ensured that enzymes with different input combinations can inhibit or promote the reaction,which lays the foundation for the design of the key in the molecular lock module.Subsequently,using the nick characteristics of the enzyme explored in the above research,a DNA molecular lock based on nanometer manipulation technology is constructed.In the DNA molecular lock,only when the correct combination of Nicking enzyme is input can the DNA molecular lock operate normally.When the wrong Nicking enzyme is input,the DNA molecular lock is considered to be out of control.At this time,the molecular lock self-destructs and cannot be opened normally.The proposed of the mechanism provides a powerful tool for information security protection.