Study On The Metabolites Of Strains Penicillium Spp. And Aspergillus Terreus

As a kind of important microorganisms,fungi have produced metabolites with diverse structures and biological activities,serving as resource of microbial natural products and pharmaceuticals.Three fungal strains were selected from more than 400 strains on the basis of antimicrobial activity and the diversity of metabolites analysis.Active isolates W70,W49 and Y6 were assigned as Penicillium sp.,Penicillium sp.and Aspergillus terreus,respectively by the analysis of their ITS-1 sequence and morphological chacteriatics.Two new compounds(1 and 2)and six known compounds were obtained from the culture of strain Penicillium sp.W70,and the structures were determined as 7-hydroxy-2,5,6-trimethyl-4H-chromen-4-one(1),fudecadione C(2),fudecadione A(3),4-hydroxy-3,6-dimethyl-2H-pyran-2-one(4),aloesone(5),2-(2'-hydroxypropyl)-5-methyl-7-hydroxychromone(6),citromycin(7)and(-)-2,3-dihydrocitromycin(8).Two new compounds(9 and 10)and 14known compounds were obtained from the culture of strain Penicillium sp.W49,and the structures were determined as 4-propyl-2,3-dihydro-1H-indene-1,2-diol(9),2-((1E,3E)-hepta-1,3-dien-1-yl)-3-methoxyphenol(10),(5Z,13E)-15-hydroxy-9-oxoprosta-5,10,13-trien-1-oic acid(11),fudecadione A(12),paxitriol(13),janthitrem B(14),3,4-dihydro-3-(2-oxo-propyl)-3,6,8-trihydroxy-1(2H)-naphthalenone(15),mikaniahumulene I(16),chryseno(17),2,4-dihydroxy-6-methylacetophenone(18),myxotrichin C(19),altechromone A(20),15-hydroxydrimenol(21),3,4-dihydro-3-(2-hydroxy-2-oxo-pentyl)-3,6,8-trihydroxy-1(2H)-naphthalenone(22),1?-hydroxy-bisabola-2,10-dien-4-one(23)and(22E)-11-acetoxy-3?,6?-dihy-dioxy-24-methyl-27-nor-9,11-seco-5?-cgolesta-7,22-dine-9-one(24).One new compound(25)and 11 known compounds were obtained from the culture of strain Aspergillus terreus Y6,and the structures were elucidated as penicillixanthone B(25),butyrolactones I(26),bisdethiobis acetylapoaranotin(27),aspergiketal(28),(3R,4R)-3,4-dihydro-4,8-dihydroxy-3-[(2R)-2-hydroxypentyl]-6,7-dimethoxy-1H-2-benzopyran-1-one(29),10-hydroxygeraniol(30),E-4-(1-propen-1-yl)-cyclopenta-1,2-diol(31),O-methylorcinol(32),terreic acid(33),4-hydroxykigelin(34),terrein(35)and 22-tetraen-3-one(36).Compounds 5,6 and 7 presented antagonistic activity against Candida albicans with MICs of 128,128 and 64?g/m L,respectively,compound 15 showed moderate antibacterial activity against Listeria monocytogenes with MIC of 64?g/m L,compounds 11 and 10 showed moderate antibacterial activity against Escherichia coli with MICs of 64 and 32?g/m L,respectively,compound 20 showed moderate antibacterial activity against Bacillus subtilis with MIC of 64?g/m L,respectively,and compounds 6 and 10 showed moderate antibacterial activity against Pseudomonas aeruginosa with MICs of 64?g/m L.Compound 14 presented cytotoxic activities against H1299 and MHCC97H tumor cells with IC₅₀ of 11.9 and 12.9?g/m L,respectively.