EDXRF Elemental Analysis Of Small Amount Of Plant Samples

Energy Dispersive X-ray Fluorescence Spectroscopy(EDXRF)has a wide range of applications in the field of plant measurement for its advantages of simple sample preparation,fast analysis,and convenient using.However,when measuring the small amounts of plant samples,the conventional sample preparation and quantitative analysis methods have been unable to perform accurate EDXRF analysis.Therefore,the development of sample preparation techniques for small amounts of plant samples in EDXRF analysis,and the establishment of quantitative analysis methods for these samples are the keys to solving the problems of EDXRF analysis in small amounts of plant samples.This paper proposes a method for rapid preparation of the thin-film plant samples by suspension filtration to solve the problem of preparation for milligram quantities of plant samples;The quantitative analysis method for these samples was established at the same time;Finally,researching the bioaccumulation of Ti O₂nanoparticles in the aquatic plant was studied.The main research contents and results are as follows:(1)The study of preparation for small amounts of plant samples.The preparation of milligram quantities of plant samples was realized by cleaning,drying,grinding,weighing,suction filtration,sample compression,drying,and packaging.The sample preparation process was optimized by the selection of the carrier filter,the selection of the particle size of plant samples,and studying the effect of sample moisture in EDXRF measurement.At the same time,the test of sample uniformity,sample repeatability,and sample stability proves that the proposed sample preparation method is uniform and reliable,which was suitable for the measurement of EDXRF.(2)The study on quantitative analysis methods of thin-film plant samples.The linear relationship between the fluorescence intensity and the deposition amount(thickness)of the thin film sample was studied by the theoretical calculation of fluorescence intensity.The calibration curve of each element in the thin film sample was established by the mass deposition,and the calibration curves of the light elements were corrected by the emission-transmission method.The linear correlation coefficient of the light element calibration curve after the correction has been improved.Three samples were synthesized to verify the accuracy of the quantitative analysis.Analytical results by EDXRF were compared with ICP-OES.In most cases,the EDXRF analysis results were closer to the true values,and the relative error range to the true values was between 1.2%and 8.5%,this indicates that the used quantitative analysis method was reliable.(3)The study on the bioaccumulation of Ti O₂nanoparticles in the aquatic plant.The proposed sample preparation method and quantitative analysis method were applied to practical research,and the bioaccumulation of Ti O₂nanoparticles in the aquatic plant,Salvinia natans were studied.The experimental results indicate that the Salvinia natans showed a strong potential to enrich the Ti O₂nanoparticles,and its bioconcentration factor(BCF)is up to 1931.5.With the accumulation of Ti O₂nanoparticles in plants,the increase of P,S and Ca elements meant that the Ti O₂nanoparticles have a positive effect on the growth and development of Salvinia natans.The reduction of Fe element indicates that the Ti O₂nanoparticles can affect the bioaccumulation of metal ions in Salvinia natans.In summary,the sample preparation method and quantitative analysis method proposed in this paper can be applied to the analysis of small amounts of plants.These methods might provide some help for the risk assessment of the contamination of the environment,and they can also be combined with other analytical methods to study of the toxicology of plants.