The Study On Modulation Of Non-classical Receptive Fields To Classical Receptive Fields In Mouse Primary Visual Cortex

Surround modulation(SM)of non-classical receptive field(n CRF)to classical receptive field(CRF)is a fundamental property of single neurons in mammalian primary visual cortex(V1).Investigating the modulation role is contribute to understand the basic principles of information integrating of large-scale image in the visual cortex.Most previous studies on SM focused on traditional visual animal,such as cat and monkey.However,the visual study on rodent has attracted more and more attention with the rapid development in optogenetics,neural tracing and other technique.Morever,recent studies shown gamma oscillations may entrained in the visual information integrating of large-scale image.Previous studies exhibited there may be significant differences in gamma oscillations induced by different SM pattern.However,there are lacking detail studies.Therefore,the thesis studies the response of spike activity and gamma oscillations(20-80 Hz)of local field potential(LFP)induced by SM role of n CRF to CRF by five different SM stimulation pattern.Our results shown:(1)It's different in suppression index(SI)of V1 neurons at different layers,in which SI of L4 neurons is greater than that of L5/6 neurons.There are no significant difference in CRF size of V1 neurons in different layers.(2)As the results in cats and monkeys,strongest SI is induced by the stimuli that the CRF and n CRF grating has the same stimulus parameter,such as spatial frequency,orientation,contrast,luminance and phase.(3)The SI of V1 neurons was highly correlated with the discontinuity sensitivity index(DSI).(4)Gamma oscillations in LFP could not be induced by flickering static grating stimulation,but low frequency(20-30 Hz)and high frequency(50-80 Hz)in gamma oscillations could be induced by drifting grating stimulation.The spike activity induced by different grating parameters was consistent with the overall trend of peak frequency energy.(5)The spike activity has a good phase-locking to the low gamma oscillations in orientation and luminance stimulus pattern,while much weaker phase-locking in spatial frequency and contrast stimulus pattern;There was also a weak phase-locking relationship between the spike activity and high frequency gamma oscillations in the four stimulation pattern.In this thesis,we analyzed the changes of spike activity,gamma oscillations and the phase-locking relationship between spike activity and gamma oscillations under different SM stimulation pattern from electrophysiological point of view,which is helpful to reveal the neural mechanism of information integrating of large-scale image,and will provide a basis for further study of SM role of n CRF to CRF using optogenetics and two-photon platform in mice.