Study On The Selective Encapsulation Of Hydrophobic Fluorescent Molecules By Amphiphilic Framework Nucleic Acid Molecule Cages

DNA not only can be treated as creatures' Genetic information but also can be a new kind of material to construct 2D or 3D nanostructure.DNA has addressability,Programmability,Predictability,and so on.In the early stage,DNA nanomaterials concentrate on the design and construction of the structure.And now it applies to other fields to solve issues in another way.There are few articles about the combination of DNA nanomaterials and molecule sieves.Some researchers studied DNA sieves.DNA has the biocompatibility to be applied in organisms.Therefore,DNA molecular sieves can screen target guest molecules in cells.However,the DNA molecular sieve sorting material only relies on the matching of the shape and size of the guest molecules and the molecular sieve channels.However,the components in the biological environment are very complex.The substances that need to be separated are not only different in size,but also charge,hydrophobicity,and groups.Therefore,this article intends to expand the mode of action that DNA molecular sieving relies on to select guest molecules.Framework nucleic acid is a type of three-dimensional structure in DNA nanomaterials,which can realize the targeted organization of nanomaterials,as a research platform or obtain new properties.Therefore,this thesis uses amphiphilic framework nucleic acids to construct a new type of DNA molecular sieve and uses hydrophobic interactions to sort molecules.The specific content is as follows:1.This thesis constructed a new DNA molecule sieve using FNA.This thesis designed a cubic FNA with 8 hydrophobic chains at 8 vertexes.These hydrophobic chains connected in the aqueous environment and formed a local hydrophobic environment.This structure was called Cube-D2.Then Cube-D2 was characterized by AFM,HPLC,and PAGE.To test that if Cube-D2 was able to adsorb substance,Cy3 was used in the experiments of agarose gel electrophoresis and NAP-5 columns.And Cube-D2 can absorb substances like molecule sieves.2.Then this thesis studied if Cube-D2 has the potential to be a new molecule sieve.The same fluorescent dyes with different modification groups were absorbed by Cube-D2.And Cube-D2 had different wrapping efficiency to these fluorescent dyes.In summary,this thesis constructed a new DNA molecule sieve that filters substance by hydrophobic interaction.This work expanded the application of DNA nanomaterials and nano molecule sieves.It has great meaning for these fields.