Incoherent Digital Holographic Sectioning With Three Dimensional Stuctured Light Illumination

Fresnel Incoherent Correlation Digital Holography(FINCH)is a wide-field 3D imaging technique.In the case of spatial incoherent light illumination or object selfluminescence,the light wave emitted from the same object point on the sample is divided into two beams by means of an appropriate wave separation method,so that the two light waves interfere with each other on the receiving plane forming a point source hologram.All point source holograms are superimposed incoherently to form a hologram of the object.The three-dimensional reconstruction can be realized by combining the hologram of the object with the numerical algorithm.Fresnel incoherent correlation digital holography technology,the lateral resolution of its imaging can break through the diffraction limit,but the axial resolution is still equivalent to traditional imaging systems.The structured light illumination technology uses Moiré fringe to encode the in-focus information,removes the de-focus information by sectioning reconstruction.Stitches all the sectioning planes can get a three-dimensional sample.Structured light illumination technology be easily combined with other imaging technologies.In order to further improve the imaging resolution and sectioning capability of FINCH,this paper introduces three-dimensional structured light illumination into Fresnel incoherent digital holography,focusing on the sectioning imaging characteristics of the FINCH system under structured light illumination.The main content of the paper as follows:1.The basic theory of incoherent digital holography for 3D structured light illumination is analyzed.The recording and reconstruction principle of incoherent digital holography is given,the generation process of three-dimensional structured light and its lateral and axial distribution characteristics are further analyzed.The principle for three-dimensional structured light illumination sectioning is demonstrated.Threedimensional structured light illumination incoherent digital holographic wide-field and sectioning reconstruction method is investigated particularly.2.The generation and optimization of three-dimensional structured light are studied.The optimized SI is applied to fluorescence digital holographic imaging.The effects of diffraction grating type,grating phase delay parameters and beam polarization on the modulation degree of the structured light in the three-dimensional direction are analyzed.Both simulated and experimental results are demonstrated in the work.With the optimized structured light illumination,the sectioning imaging characteristics of the three-dimensional structured light illumination fluorescence digital holographic imaging system are also studied experimentally.3.Exploring another algorithm of structured light illumination section reconstruction.Integrated-SIM algorithm is employed to reconstruct the hologram sectionally.The characteristics of Integrated-SIM used for 3D structured light illumination incoherent digital holographic sectioning reconstruction are analyzed by simulation.The feasibility of integrated-SIM for section is verified by two-dimensional structured light illumination incoherent digital holography system in experiment.Integrated-SIM The section imaging ability of three-dimensional structured light illumination incoherent digital holography combining Integrated-SIM reconstruction is discussed.