Study Of The Laser-induced Forward Transfer For Preparation Of Protein Microarray

The laser-induced forward transfer technique(LIFT)based bio-printing technology(also known as biological laser printing,Bio LP)allows depositing picoliter volumes of solutions containing biomolecules in precise positions without alteration of their biological activity,is an emerging bio-printing technology.Due to the technology is characterized by non-contact,good direction,the high laser energy density,which overcomes the shortcomings such as low resolution,nozzle/pinhole easy to jam,samples easy to pollution of conventional microarray preparation methods like pin deposition or inkjet printing,make it highly competitive in front of more conventional techniques.This article described in detail the principle / devices of the laser-induced forward transfer printer used for protein microarray fabrication,prudently exploded the key of LIFT printing process and the impact of print results while altering related parameters.Then,we built some protein microarrays structure which applied to heterogeneous immunoassays,to explore new mechanisms and strategies of proteomics.In this study,firstly,we introduced the domestic and international status of biological laser printing technology,microarray preparation,and analyzed the possibility and great significance of introducing biological laser printing into protein microarray production.Follow that,we displayed the bio-laser printer and introduced its system composition and functions.Then,according to the characteristics is required of the laser absorption layer in biological laser printing technology,we chose the titanium coatings as the absorption layer.There were four different methods used for preparing a 50 μm～100 μm titanium film on a glass slide.We made a systematic research about the properties of film,like surface morphology,structure and crystalline,ultimately,we got the better method for preparing the titanium film in biological laser printing technology and more suitable treatment technology to form an uniform bioink film.More,we used the built biological laser printing platform to explore protein microarrays printing.Different parameters including the solution viscosity,the thickness of the bioink film,modification methods of receiving biological substrate was set in printing process to explore the impact of these parameters on the print results and analyzed the relationship between changes of parameters and deposition point size.It was found that the solution viscosity and the liquid film thickness were varied and droplets as small as 15 μm in diameter were obtained.Finally,under the condition of ideal parameters,a human Ig G microarray of 65 μm in diameter was printed by biological laser printer and had a heterogeneous immune response with Cy5 fluorescently labeled Ig G after BSA blocking,then the activity of human Ig G could be detected by inverted fluorescence microscope after printing.The results showed that the human Ig G microarrays transferred by biological laser printer remains active and could be used in heterogeneous immunoassays.In addition,we combined this printing technique with CAD software,made a further study of applications of biological laser printing in Biomaterial patterning as we created some special letter-like micro-array patterns.All have shown that,something like laser-induced forward transfer technique(LIFT)based bio-laser printing technology combined with Biomaterial patterning for building protein microarrays have great value in life science.