Structure Analysis Of Medium-Long Chain Triglyerides (MLCT) And Identification Of Triglycerides In 12 Kinds Of Vegetable Oil

The methods of structure analysis of medium-chain triglycerides and identification of triglyceirdes in 12 kinds of vegetable oil were established in this research.MLCT structure analysis mainly comprised fatty acid composition,triglyceride annlysis andβfatty acid composition.Triglyceride was identified by HPLC-APCI/MSD utilizing silver ion column in series in 12 kinds of vegetable oil.Transmethylesterification and GC-FID were used in the method of fatty acid composition which was a fast test method with less process and no heat.Triglyceride was analyzed by HTGC-FID was estabilished,and triglycerides in MLCT was elicited with fatty acid composition and retention time.GC-QTOF was utilized in quanlitive method ofβfatty acid composition with grignard regent method,andβ-MAG was proved to be appeared and retention time was confirmed,andα-MAG was absent.The condition test was developed in this research and the experiment condition,such as enzyme amount,alcoholysis reaction temperature,alcoholysis reaction time and silylating reagent volume were optimized by single factor test.β-C8-MAG,β-C10-MAG,β-C12-MAG,β-C14-MAG,β-C16-MAG andβ-C18-MAG were determinated in MLCT.Identification of triglycerides in 12 vegetable oils by HPLC-APCI/MSD utilizing silver ion culomns in series were also estabilished in this research.Difference of retention time of ion peak,numbers of double bonds,[DAG]~+ions,and uneverness of triglycerides were used to identify the type of triglycerides.Some characteristic oils,such as groundnut oil,sunflower oil,rapeseed oil and low erucic acid rapeseed oil,could be recognized by the identification of characteristic triglycerides.