Preparation Of Molecularly Imprinted Polymers And Application In Electrochemical Sensing

Paclitaxel(PTX)is clinically used for the treatment of lung cancer and other malignant tumors.At present,PTX is mainly extracted from taxus and obtained by semi-synthesis.The most part of the cost for PTX is concentrated in the purification process.Therefore,it is of great significance to develop an efficient and low-cost purification method.Aspartame(ASP)is a kind of sweet food additive,which is reported to be harmful to the body.And it is important to explore a sensitive and convenient analysis method to monitor the dosage.The present study was trying to solve the above problems through the molecularly imprinted polymers(MIPs).Deep eutectic solvent(DES)is a solvent that can be formed between a hydrogen bond donor and a hydrogen bond acceptor.DES has many attractive properties,including simple synthesis,low cost,low toxicity,and easy bio-degradability,so it can be widely used in organic catalysis,electrochemistry,nanomaterials and biochemistry fields.In this paper,DES was used as a functional monomer to synthesize different types of MIPs materials,which can be applied as solid phase extraction,drug carriers,electrochemical sensing and et al..Firstly,using zein as a cross-linking agent and DES as a functional monomer,a highly efficient and sensitive magnetic imprinted polymer(ZDM-MIPs)was prepared.This kind of polymer can be used to remove ASP from drinks,and the magnetic glassy carbon electrode modified with ZDM-MIPs materials can be used to detect ASP level in food.The results showed that in the concentration range of 0.1-50 μg/m L,the ZDM-MIPs magnetic glassy carbon electrode had a good response to ASP,the recovery rate was from 84 to 107%.Secondly,DES was used as the functional monomer to prepare PTX molecularly imprinted polymers,which was then used to separate and enrich PTX.Adsorption experiments showed that the maximum adsorption capacity and optimal adsorption time of this DES-MIPs were 87.08 mg/g and 180 min,the maximum selectivity coefficient was 6.20,for which the stability and repeatability were excellent.Finally,using 10-DAB and paclitaxel side chain as dummy template molecules,two different dummy molecularly imprinted polymers 10-DAB-MIPs and SC-MIPs were prepared for enriching PTX.The 10-DAB-MIPs and SC-MIPs materials loaded with PTX were co-incubated with biological samples to investigate the release behavior of PTX.The adsorption capacity were 23.58 mg/g and 21.64 mg/g,respectively,and a large amount of PTX was detected in the biological samples with elution rate about 45%.Research results showed that these two different materials have potential application value as drug carriers in biological systems.