Preparation Of Lycopene And Lycopene Liposomes From Supercritical Fluid Extraction

Lycopene is a kind of natural carotenoid,with multi-function,can quenchreactive oxygen species,human body free radicals can be eliminated by it,it can make people slow down the occurrence of atherosclerosis,prevent a variety of lesions carcinogenic,protect the aging of cardiovascular and cerebrovascular.Lycopene is insoluble in water,sensitive to light and temperature,easy to be oxidized and decomposed and cis-trans isomerism,poor stability,not suitable for human absorption,so its bioavailability is low,the application of a great limitation.In this paper,the technical conditions for extraction of lycopene by supercritical CO₂method were studied.The extracted lycopene was used as the core material,and the preparation of lycopene nano-liposomes by film-ultrasonic method was used to improve the water solubility and stability of lycopene.The extraction processes of lycopene mainly include organic solvent extraction,supercritical CO₂ extraction,HPLC,enzyme,microbial fermentation and direct comminution.However,the traditional extraction of lycopene has many disadvantages,such as large solvent loss,long time consumption,low yield and purity,low selectivity,and high safety risk.The supercritical CO₂ extraction method has the advantages of high selectivity,short extraction time,non-toxic extraction solvent and high extraction rate.This topic tomato powder prepared by freeze drying method,use of lycopene extraction by supercritical CO₂ extraction technology to optimize process conditions,and select natural soybean phospholipid as the main membrane material,establish the optimum process conditions of lycopene liposomes,after analysis of lycopene in interaction with phospholipid,liposome for the feasibility study of liposoluble sexual factor embedding provide theoretical guidance.The stability of lycopene nano-liposome was evaluated at the same time.First,the optimal extraction conditions for supercritical CO₂ of lycopene were as follows,the extraction pressure,modifier flow rate,CO₂ flow rate,extraction temperature and extraction time was 35 MP,5 m L/min,6 m L/min,46?and 90 min,respectively.The optimal extraction rate of lycopene was 90.21%and the lycopene content of tomato powder was3648.57?g/g,the extraction rate of lycopene was higher,the optimization effect was better,and it had good practical application value under the optimal conditions.In addition,the optimal conditions for the preparation of lycopene liposomes by thin-film ultrasound were as follows,the PH value of PBS buffer solution,the ratio of phospholipid to cholesterol,the optimal ratio of lycopene to phospholipid,the optimal ultrasonic time and the optimal temperature was 7.4,4:1,1:100,4min and 30?,respectively.The liposome was spherical with uniform size,and the encapsulation rate of lycopene was 96.38%under optimal conditions.The average particle size of lycopene liposome was 123.5 nm and the PDI was 0.24±0.02by dynamic light scattering method,with small particle size and good dispersion.The micromorphology and morphology of lycopene liposomes must be observed by transmission electron microscopy.Lycopene liposome vesicles formed into regular spheres and were evenly distributed under electron microscope.In addition,the successful construction of lycopene liposomes was verified by infrared spectroscopy,and the changes of particle size and retention rate of lycopene liposomes during storage were studied.The results showed that the particle size and retention rate did not change significantly under the condition of 4?,while the particle size and retention rate changed significantly under the condition of 25?.