Nanocrystalline Cellulose Coated Magnetic Nanoparticle Used For Glycoprotein's Affinity Adsorption

Glycoprotein is a complex protein composed of a branched oligosaccharide chain and a polypeptide chain covalently connected.The main chain is short.In most cases,the protein is more than that of carbohydrate content.At the same time,glycoprotein is a binding protein.It is a molecule composed of a short oligosaccharide chain and a protein covalently linked.In this experiment,a new core-shell nanomaterial based on Fe3O4 nanoparticles and 2,3-dialdehyde-based cellulose(DAC)coating was introduced.And the performance in the efficient adsorption and separation of glycoprotein application was mentioned.Oxidation of nanocellulose using sodium periodate as an oxidant yields aldehyde-based cellulose,which can effectively provide sufficient aldehyde-based binding sites.Firstly,a surface of Fe3O4 nanoparticles is coated with a layer of DAC,and then 3-aminophenylboronic acid(PBA)is connected to the DAC through an aldehyde group-amino reaction to form a PBA-functionalized core-shell magnetic nanomaterial.The material has a strong adsorption capacity for glycoproteins.In addition,the concentration of glycoproteins by nanomaterials can be triggered by changing the pH value without destroying these structures.Scanning electron microscopy(SEM),F ourier-transform infrared(FT-IR)spectroscopy,X-ray photoelectron spectroscopy(XPS),and X-ray diffraction(XRD)were utilized to study the magnetic nanoparticles.Ultraviolet,fluorescence,infrared and other spectrophotometers and gel electrophorese is for qualitative and quantitative analysis of materials in protein applications.The experimental results show that due to the participation of cellulose,these nanocomposites are effective tools for selective and efficient enrichment of glycoproteins.The synthesis reaction conditions of 2,3-dialdehyde cellulose were set at 17h,45?,and the oxidant concentration was 25g/L.The oxidation efficiency reached the optimal value of 71%.Then switching between alkaline(pH=8.5)and acidic conditions(pH=4.5),Fe3O4@DAC-PBA magnetic nanomaterials maintain high adsorption capacity for glycoproteins(9.65 mg/g,recovery rate 94.2%),and the material can be reused three times during the glycoprotein enrichment experiment.The use of 2,3-dialdehyde cellulose provides more binding sites for the connection of 3-aminophenylboronic acid and improves the stability of the material,ensuring the excellent magnetic properties of Fe3O4@DAC-PBA nanocomposite and ensuring glycoprotein Good magnetic response during adsorption and separation.It should be pointed out that this Fe3O4@DAC-PBA magnetic nanocomposite provides new ideas and applications in various fields such as biomedicine in the future,food safety and even material synthesis.