Determination Of VB₆ In Food By Liquid Chromatography Based On Magnetic Nanomaterials

As an important nutrient element of VB₆ is closely related to human health,it is particularly important to detect its content quickly and efficiently.Magnetic nanomaterials have the advantages of good biocompatibility and easy to be functionalized,so they can be well used in sample pretreatment.Currently existing in vitamin testing before dealing with complex,time-consuming and low detection efficiency problems,this study magnetic nanomaterials as a starting point,respectively synthesized function of ion pair reagent 1-butyl 3-methyl imidazole magnetic ferroferric oxide materials and on the basis of the mesoporous materials of magnetic nano mesoporous materials,and using the synthetic materials to establish a new method for the detection of VB₆.The main research contents and results are as follows:（1）Synthesis and characterization of 1-butyl 3-methyl imidazole magnetic ferric oxideUsing ferric chloride and ferrous sulfate as materials,the magnetic nano-sized ferric oxide was modified with 1-butyl 3-methyl imidazolium bromate as ionic pair reagent.Under the acidic environment provided by acetic acid,the magnetic nano-sized ferric oxide was successfully synthesized.The effects of time,temperature and feed ratio on the adsorption performance of 1-butyl 3-methylimidazole magnetic ferrooxide were studied.Finally,the synthesis time was determined as 12 h,ultrasonic 4 h,acetic acid 200 m L,1-butyl 3-methylimidazole bromate 300 mg.The dispersion of the material in solution was observed,and it was found that the material had good dispersion and could be separated quickly in an external magnetic field.The size distribution was found to be uniform under transmission electron microscope.FT-IR spectra showed that 1-butyl 3-methyl imidazole magnetic Fe₃O₄ was successfully synthesized.Through data fitting adsorption isotherm,adsorption kinetics and thermodynamic analysis,it is found that physical adsorption and ion exchange adsorption exist in the reaction process,and the adsorption process is exothermic adsorption.The subsequent stability and reusability experiments show that the material can be recycled.（2）Synthesis and characterization of magnetic nano-mesoporous materialsNano-mesoporous materials were synthesized by using poloxam reagent,3-aminophenol and hexamethylenetramine as materials under magnetic stirring.The precursors were mixed with iron powder in a certain proportion and added with ethanol reagent.Nano-mesoporous materials were synthesized by ultrasonic calcination.The effects of time,temperature and feeding ratio on the adsorption performance of nano-mesoporous materials were studied.Finally,poloxam reagent was determined to be 5 g,3-aminophenol 2.5 g,2.0 g hexamethylenetetramine.Observe the dispersion of the material in the solution found that the material dispersion is good,in the outside Add magnetic field can practice fast separation;The size distribution was found to be uniform under transmission electron microscope.XRD shows that the crystal shape of the material is consistent with that of Fe3O4.FT-IR spectra and thermogravimetric analysis also showed that magnetic nano-mesoporous materials were successfully synthesized.Through data fitting adsorption isotherm,adsorption kinetics and thermodynamic analysis,it is found that physical adsorption and ion exchange adsorption exist in the reaction process,and the adsorption process is exothermic adsorption.The subsequent stability and reusability experiments show that the material can be recycled.（3）A method for the determination of VB₆ in banana by liquid chromatography based on magnetic nano-mesoporous materials was developedA HPLC method for the determination of VB₆ in banana was established.The method could complete the separation and determination of VB₆ in 6 minutes.The results showed that the linear range of VB₆ in banana was 0～20.0μg/m L,the limit of detection was 0.03μg/100 g.The method has high detection efficiency and can save a lot of time.The adsorbent is easy to synthesize and the detection cost can be reduced.