| Objective:Compare the content of different elements in Cyclocarya paliurus by different digestion methods;develop Cyclocarya paliurus Zinc-Calcium Oral Liquid;develop hard capsules of Cyclocarya paliurus and Aloe Hypoglycemic Formula and conduct quality inspection and quality control;develop Cyclocarya paliurus beverage and in vitro functionl evaluation.Methods: 1 Development of Cyclocarya paliurus zinc supplement oral solution 1.1 Research on the content of beneficial elements in Cyclocarya paliurusIn this experiment,three pre-treatment methods,wet digestion method,dry digestion method and graphite digestion method,were selected.ICP-OES was used to simultaneously determine the contents of six beneficial elements in Cyclocarya paliurus tea,and methodological verification was performed.Difference in element content.1.2 Animal experiment of the effect of Cyclocarya paliurus supplementation of zinc and calciumThe effect of Zinc and Calcium supplementation on the water extract of Cyclocarya paliurus was studied.The Zinc and Calcium deficient model was made with Zinc and Calcium deficient feed.The zinc-calcium deficiency model was supplemented by gavage,and the zinc-calcium supplementation was evaluated with the corresponding evaluation index effect.2 Development of Cyclocarya paliurus hypoglycemic capsule 2.1 Optimization of water extraction processIn this test,the extraction rate and polysaccharide content were used as the evaluation indicators.The extraction time,extraction temperature and material-liquid ratio were selected as the investigation factors.The best extraction process was obtained through single factor test and orthogonal test and verified.2.2 Investigation of concentration temperatureTake 5 filtrates of the green money willow extract,and use a rotary evaporator to select 40 ° C,50 ° C,60 ° C,70 ° C,80 ° C,and 90 ° C to concentrate to 60 ml,and record the concentration The time and determination of the polysaccharide content of the concentrate were compared.2.3 Selection of molding processMix the Cyclocarya paliurus tea extract powder and aloe powder at a ratio of 1: 1 by weight of the raw material,then mix the mixed powder with the auxiliary materials to granulate,and evaluate according to the index of the particles.The screening of drugmaterial ratio,auxiliary material ratio and wetting agent concentration finally determines the best molding process.2.4 Quality inspection and quality controlThe quality of Cyclocarya paliurus hypoglycemic capsules was inspected for differences in appearance,moisture,disintegration time,and loading differences.A method was established to identify quercetin and kaempferol by TLC and determine the content of quercetin and kaempferol by HPLC.3 Development of Cyclocarya paliurus unilateral beverage 3.1 Optimization of water extraction processIn this test,the polysaccharide yield and flavonoid yield were evaluated as indicators.The extraction time,extraction temperature,and material-liquid ratio were selected as the main factors.The single factor test,the mixture design test,and the verification test were used to test the water of Cyclocarya paliurus unilateral beverage.The extraction process is preferred.3.2 Research on the blending processTaking the taste,color,odor,and tissue state as evaluation indicators,adding appropriate amount of stevioside and citric acid for blending,the blending process of Cyclocarya paliurus unilateral beverage was optimized.3.3 Evaluation of Antioxidant FunctionThe Cyclocarya paliurus unilateral beverages were prepared in different concentrations,and free radical scavenging experiments were carried out on samples of various concentrations to evaluate the antioxidant capacity of the unilateral beverage.Results:1 Six kinds of human essential nutrients are abundant in Cyclocarya paliurus tea.Among them,the average values of the contents of the constant elements K,Ca,and Mg were 45444,14344,and 10122 mg · kg-1,all of which exceeded 10 000.0 mg · kg-1,and the contents of the trace element Mn were 837 mg · kg-1,Zn,Cu.The contents were 50.4 mg · kg-1 and 12.5 mg · kg-1,respectively.The order of the average content of each element is: Ca> K> Mg> Mn> Zn> Cu.Experiments on the effects of zinc supplementation on the water extract of Cyclocarya paliurus were carried out.As a result,the model effect of zinc deficiency model is not ideal.It is speculated that the different feed formulas may be different.The effect of administering zinc and calcium supplements is not ideal.It is speculated that the composition of the water extract of Cyclocarya paliurus may be complex.Other medicinal ingredients inhibit or even mask the efficacy of zinc and calcium supplements.2 The best water extraction process of Cyclocarya paliurus jiang Jiangtang Capsule is: extraction time 1.0h,extraction temperature 90 ° C,material-liquid ratio 1:40;the concentration temperature of the water extraction liquid is 60 ° C,at this time it needs 60 m L to concentrate 600 m L Minutes;extracts can be easily dried by freezedrying method;the best molding process of Cyclocarya paliurus Liu Jiangtang formula is: the main drug,microcrystalline cellulose and lactose are mixed at a ratio of 20: 5: 9 by weight,and a 50% ethanol solution is used As a wetting agent;after inspection,the capsules produced all meet the requirements of the 2015 edition of the "Chinese Pharmacopoeia" Part IV(General Rule 0103)for capsules;a qualitative and quantitative inspection method for the capsules was initially determined.3 Cyclocarya paliurus unilateral beverage uses polysaccharide yield and flavonoid yield as evaluation indicators.The best theoretical technological parameters obtained are: extraction time 1.85 h,extraction temperature 96.4° C,material-liquid ratio 1:42.5 The rate was 2.34%,and the flavonoid yield was 2.51%.The best blending process is: steviol glycoside 0.015%,citric acid 0.012%,the sensory score of the beverage is the highest,and the product has the best sweet-acid ratio.The beverage has been evaluated for its antioxidant function in vitro,indicating that it has antioxidant capacity.Conclusion:1 Different digestion methods have little effect on the element content of Cyclocarya paliurus,and the specific digestion method should be determined according to the sample and the type of the tested elements;2 The preparation process of Cyclocarya paliurus hypoglycemic capsules was preliminarily determined,and the quality inspection,qualitative and quantitative inspection of hard capsules were carried out;3 The water extraction and blending process of Cyclocarya paliurus unilateral beverage was optimized and the antioxidant experiment confirmed that the beverage had obvious antioxidant effect. |
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