The Interaction Of Different Aspect Ratio Gold Nanorods On Ovarian Cancer Cells Based On Optical Imaging

At present,the incidence of malignant ovarian tumors is the second highest in the world.The incidence rate of ovarian cancer is the highest in the world.The mortality rate is the highest in gynecological tumors.The cause of ovarian cancer is complex.There is no obvious characteristic when the disease begins.At present,the current medical conditions can not detect the early patients.Therefore,the research on screening for early ovarian cancer is more urgent and important.In recent years,the research of gold nanomaterials in biomedical applications has attracted extensive attention.Among many gold nanomaterials,gold nanorods,with their unique chemical and physical properties and simple preparation process,have high application value in many biomedical fields,such as medical imaging,photothermal therapy,biological detection and so on.In this study,we focused on the interaction between cells and gold nanorods with unique optical properties.The toxicity of different sizes of gold nanorods to different cells,the uptake and clearance of different sizes of gold nanorods by different cells,and the distribution of gold nanorods after entering different cells were studied by comparing human normal ovarian epithelial cells(HOSEpi C)and ovarian cancer cells(HO-8910).In addition,the effect of protein crown on the internalization of gold nanorods was also investigated.The results showed that the toxicity of gold nanorods to cells and the uptake of gold nanorods by cells were affected by many factors.The toxicity of gold nanorods to cells is related to the size of gold nanorods and the presence of serum in the medium.The toxicity of gold nanorods to cells is inversely proportional to the ratio of length to diameter of gold nanorods.The smaller the ratio of length to diameter is,the greater the toxicity of gold nanorods to cells is.When serum is added to the culture medium,the serum protein forms a protein crown around the gold nanorods,and the presence of protein crown significantly reduces the internalization ability of gold nanorods.The GNRs-700 nm gold nanorods with the shortest ratio of length to diameter were the most absorbed by the two kinds of cells,but there was no significant difference between the GNRs-760 nm and GNRs-820 nm gold nanorods.For the two types of cells investigated,the location of gold nanorods with different length to diameter ratio after entering different cells is the same.The three kinds of gold nanorods enter the cells first to the early inclusion body,then to the late inclusion body,then to the mitochondria,and finally to the lysosome.However,in the early stage of gold nanorods entering the cells,the uptake of gold nanorods by different cells exists for a short time The results showed that different ratio of length to diameter of gold nanorods into HOSEpi C and HO-8910 cells was mediated by different receptors.The exocytosis process of HOSEpi C and HO-8910 cells to the gold nanorods with different length diameter ratio is basically the same,but the exocytosis rate of the cells is inversely proportional to the length diameter ratio of the gold nanorods,and the shorter the length diameter ratio,the faster the exocytosis rate.The research will provide the research foundation for the application of gold nanorods in the diagnosis and treatment of ovarian cancer in the future.