| Electrochemiluminescence（ECL）,as a new detection technology,is often used for quantitative analysis of various drugs,macromolecular proteins,and inorganic small molecules.Among them,electrochemical sensors have become a research hotspot in environmental safety monitoring due to their advantages such as fast analysis speed,simple operation,and low cost.The development of new functional nanomaterials is a key step in the development of high-performance electrochemical sensors.The two-dimensional Ti3C2 MXene material exhibits excellent performance in electrochemical sensing due to its large specific surface area and good metal conductivity.Moreover,the Ti3C2MXene could couple with other materials to broad the application prospects in the field of electrochemiluminescence.In this paper,two-dimensional Ti3C2 MXene materials are loaded with different electrochemiluminescence materials to prepare a series of Ti3C2MXene-based functional nanomaterials,and nucleic acid aptamers are introduced to improve their selectivity.Based on these Ti3C2 MXene-based materials,different electrochemiluminescence aptasensors are developed for detect antibiotics in actual samples.These method are simple,rapid,selective,and have achieved certain research results.The specific contents are described as follows:1.Silver bromide（AgBr）-titanium carbide（Ti3C2）MXene-based electrochemiluminescence aptamer sensor for detection of enrofloxacinIn this study,AgBr-Ti3C2 composite material with excellent electrochemiluminescence performance was developed based on a wet chemical method.Then enrofloxacin aptamer is immobilized by electrostatic adsorption.Based on the mechanism of enrofloxacin（ENR）enhancing the ECL signal of aptamer/AgBr-Ti3C2 modified glassy carbon electrode,a highly sensitive and a new ECL aptamer sensor was fabricated.The sensor has good detection performance including a wide linear range and a low detection limit.Under the best experimental conditions,the ECL signal has a linear relationship with the logarithm of the enrofloxacin concentration,and its linear range is1.0×10-12mol/L～1.0×10-6 mol/L with the low detection limit(5.92×10-13mol/L,S/N=3).The as-fabricated ECL aptasensor could use to detect enrofloxacin in pond water.2.Silver phosphate（Ag3PO4）-titanium carbide（Ti3C2）MXene-based electrochemiluminescence aptamer sensor for detection of lincomycinIn this study,Ag3PO4 was used as the luminous body and loaded on thin-layer Ti3C2 nanosheets to prepare Ag3PO4-Ti3C2 composite materials with excellent electrochemiluminescence performance.With the assistance of lincomycin aptamer,a new ECL aptasensor was successfully constructed.Under the best experimental conditions,the ECL signal has a linear response to the logarithm of the concentration of lincomycin,and its linear range is1.0×10-13 mol/L～1.0×10-7 mol/L,and the detection limit is 6.17×10-14 mol/L（S/N=3）.The as-fabricated ECL aptasensor is suitable for the detection of lincomycin content in aquaculture wastewater.In addition,the sensor exhibits excellent selectivity and stability.3.Detection of chloramphenicol by utilizing zinc oxide quantum dots（ZnO）/nitrogen-doped Ti3C2 MXene-based electrochemiluminescence aptamer sensorIn this study,a heat treatment method was used to synthesize zinc oxide quantum dots/nitrogen-doped titanium carbide nanomaterials（ZnO/N-Ti3C2）,and at the same time,the nitrogen content was adjusted by tuning the amino acid dosage.Based on the mechanism that chloramphenicol can enhance the ECL signal of aptamer/ZnO/N-Ti3C2 modified glassy carbon electrode,an ECL aptamer with good stability and selective recognition of CAP has been successfully constructed.Under optimized conditions,the enhanced value of the sensor’s ECL signal is proportional to the concentration of chloramphenicol.The logarithm has a linear relationship with a linear range of 0.1-100 ng/mL and a detection limit of 0.019 ng/mL（S/N=3）,and it is suitable for the detection of chloramphenicol content in fish pond water. |
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