Research On The Detection And Application Of Respiratory Disease Related Biomarkers With Biosensing Platform Based On Nanomaterials

Disease biomarkers are a class of measurable and assessable biological indicators,which can be used for early screening,predicting and diagnosing of diseases,as well as monitoring the treatment process of drug intervention.The ideal biomarkers should have the characteristics of satisfactory specificity,excellent stability and non-invasiveness,which make them star in the field of disease diagnosis.As we all know,Respiratory-related diseases are the most common disease,and the abnormal expression of their biomarkers is greatly related to lung injury.Therefore,it is very critical to find ideal disease biomarkers,which can not only improve the diagnosis rate,but also give individualized treatment as soon as possible.Recently,the electrochemical biosensors were widely used owing to quick,convenient,sensitive and cost-effective merits.Hence,in our works,we designed miRNA biosensor and aptasensor for the detection micro RNA of Idiopathic pulmonary fibrosis（IPF）and ESAT-6antigen of Tuberculosis（TB）,respectively.The research contents were as follows:1.An efficient electrochemical assay for miR-3675-3p in human serum based on the nanohybrid of functionalized fullerene and metal-organic framework.Firstly,a new nanohybrid was successfully synthesized via phase transfer method that C₆₀was doped into poly（amidoamine）（PAMAM）-functionalized metal-organic framework,exhibiting salient redox activity compared with NC₆₀@PAMAM-MOF prepared via direct-mixing method.Furthermore,their redox activity was higher than that of C₆₀@PAMAM in the absence ofMOF.Secondly,acetylene black（AB）modified with core@shell Au@Pt nanoparticles were employed as a sensing platform to amplify the detection signal further.Finally,the miRNA biosensor showed a linear relationship at the range from 10 fM to10 nM with a limit of detection is 2.99 fM.More importantly,the designed biosensor possessed more suitable detection performance compared with qRT-PCR,illustrating that this method provided a new way for the diagnosis of IPF.2.Cognate-aptamer-based biosensor using PANI-doped MXene-AB coupled with 3D flower-like NML@N-CNTs nanocomposite for ESAT-6antigen detection.First of all,polyaniline（PANI）was doped with MXene-AB to obtain a new multilayer nanocomposite（MXene-AB-PANI）,which can enhance conductivity and load more gold nanoparticles（AuNPs）and aptamer Ⅱ（EBA Ⅱ）to obtain a signal marker.Next,A 3D flower-like NML@N-CNTs nanocomposite was successfully prepared via electrostatic interaction between NiMn LDH（NML）and nitrogen-doped carbon nanotubes（N-CNTs）,showing excellent dispersion and more active sites to load ESAT-6 aptamer Ⅰ（EBA Ⅰ）.Ultimately,the ESAT-6 antigen was sandwiched and quantitatively detected in the range of 10 fg/mL to 100ng/mL.Furthermore,the as-prepared aptasensor showed good stability and specificity,and obtained satisfactory results in clinical serum detection.