Study On The Preparation And Efficacy Of Fermentation Broth Of Pomegranate Peel And Schisandra Chinensis

Pomegranate is rich in ellagic acid,flavonoids and other antioxidants,while Schisandra Chinensis is rich in lignans antioxidants.Both of them have been widely studied and applied in food and drug fields.In this paper,the probiotic fermentation process of pomegranate peel and Schisandra Chinensis（PP&SC）was optimized and evaluated so as to prepare a fermentation broth with good antioxidant performance.The research results were as follows:（1）Using pomegranate peel and Schisandra Chinensis as raw materials,the optimal fermentation process conditions were obtained by single factor test and response surface method as follows:1:1（w/w）ratio of pomegranate peel to Schisandra Chinensis,1%（v:v）of strains with 1:1（v:v）ratio of Lactobacillus Plantarum to Streptococcus Thermophilus,37℃of fermentation temperature and 33 h of fermentation time.（2）Through the dynamic monitoring of Tannin and ellagic acid content and the scavenging ability to ABTS,DPPH,OH^-and O₂^-of the fermentation broth,it was found that ellagic acid content and the scavenging ability to four kinds of free radicals changed with a S-typed trend.（3）Through the efficacy evaluation at the cell level,it was found that the fermentation broth and water extract could promote cell growth at the concentration of 0.5 mg/m L to 6mg/m L,and the water extract had stronger cytotoxicity than the fermentation broth at the concentration of 10 mg/m L to 15 mg/m L.For Ha Ca T cells,concentration of fermentation broth was 4～8 mg/m L has a good protective and antioxidant effect,and can effectively reduce the ROS level in Ha Ca T cells damaged by H₂O₂.For B16 cells,concentration of fermentation broth was 2～8 mg/m L has inhibitory effect on melanin.