Synthesis Of Perylene-Cyclodextrin-Carbohydrate Conjugates And Their Functionalization

Multivalent carbohydrate ligands played an important effect on the biological process,such as the protein-carbohydrate interactions and the carbohydrates-carbohydrates.The synthesis of multivalent ligands can be used to interfere with the biological identification process caused by the carbohydrates based ligands.Based on the biological activity and targeting of sugar,the self-assembling behavior and good optical properties of perylene bisimide derivatives,combined with the inclusion capacity of the cyclodextrin cavity,water-soluble perylene bisimide-cyclodextrin-carbohydrate conjugates were synthesized.In them,carbohydrate molecules were not only as the water soluble groups,but also as the recognition sites,perylene bisimides were as the effective fluorescence reporting group and self-assembling backbones,cyclodextrins were as the drug molecule inclusion sites.Their optical properties were investigated by UV visible spectroscopy(UV-Vis),fluorescence(FL)spectroscopy and circular dichroism(CD)analyses.Furthermore,their photodynamic therapy effect against MCF-7,A549,Hela and Hep G2 cells based on compounds PBI-2TMCD-Man and PBI-2TMCD-3Man,and the drug delivery properties based on compounds PBI-2TMCD-Man and PBI-2TMCD-3Man with camptothecin were studied.Compounds PBI-2TMCD-Man,PBI-2TMCD-3Man,and the inclusion complexes of compounds PBI-2TMCD-Man and PBI-2TMCD-3Man with camptothecin showed potent anticancer activities.