Preliminary Study On Anti-oxidant Activities Of Total Flavonoids In Extracts From Sanxian Tang And Preparation Of Their Oral Liquid And Quality Control

Sanxian tang consists of agrimoniae herba,epimedii folium and curculiginis rhizome,which has the functions of support right and supplement vacurity,nourishing the liver and kidney,strengthening spleen.Because these three medicinal herbs have excellent quality and reasonable price,rich source and significant efficacy,they are substituted for ginseng radix et rhizome in the prescriptions.Up to now,there are few literature studies on anti-oxidant activities,preparation and quality control of sanxian tang.The traditional decoction decocts difficult,tastes bitter and astringent,takes a great dose,makes it inconvenient to carry,and cannot be stored for a long time.In order to overcome the above shortcomings, regardless of basic research or applied research,For better development and utilization,It is necessary to do preliminary study on anti-oxidant activities of total flavonoids in extracts from sanxian tang and preparation of their oral liquid and quality control.In order to provide the theoretical basis for its further development and popularization,We will optimize the extraction process of total flavonoids from sanxian tang,preliminarily study on free radical scavenging activity of their extracts,preparation of their oral liquid and quality control.In the preparation process,to determine the best extraction process and the most reasonable formulation process,based on the total flavonoids,We had Made systematic study on ultrasonic extraction process by single-factor test and orthogonal test,The amount of flavoring agents,preservatives and pH of oral liquids was also preliminarily investigated.In scavenging free radicals,the antioxidant activity was evaluated based on the scavenging capacity against DPPH·,·OH,O₂^-·and reducing power of Fe³⁺.In controlling quality,we had identified agrimoniae herba,epimedii folium and curculiginis rhizome by TLC,established a method of determining total flavonoids from sanxian oral liquid by UV method.carried out quality testing of character,relative density and pH with reference to mixture in2015 Edition Ch.P.four part.