Synthetic Design Of PH NIR Fluorescent Probe And Its Application In Animal Model Of Chronic Wound

This paper mainly introduces the application of near-infrared fluorescence imaging technology in monitoring the pH changes in the course of chronic wounds development.Chronic wounds are in alkaline environment(7.15～8.93),which is in turn not conducive to the healing process.Chronic wounds,have high incidence rate,are a public health care problem with common types of diabetic foot ulcers,venous leg ulcer,pressure ulcers,etc.The number of patient suffering from chronic wounds is striking and a huge amount of health care expenditure is spent on chronic wounds in each country every year.Thus,it is of great clinical significance for determination of wound bed condition to monitor the pH changes in the course of chronic wound development.Currently,pH measurement tools used in most studies are not suitable for real-time dynamic pH monitoring of chronic wound bed.In addition,it has high demand of equipment and technician to operate those pH measurement tools,which makes their widespread application in clinical practice impossible.Near-infrared fluorescence imaging technology has been widely used in biomedical science due to its advantages of high spatial and temporal resolution,minimal photodamage to biological samples,high signal-to-noise ratio,simple operation,etc.Here,we applied the near-infrared fluorescence imaging technology to the pH monitoring of chronic wound bed for the first time.A kind of pH-sensitive near-infrared fluorescent probes with alkaline p Kas were constructed by introducing NIR cyanine dye IR-780 as the fluorescence reporter group and hydrazide group as the proton binding site.We obtained a near-infrared fluorescent probe,named as Alka P-1,with an appropriate p Ka of 8.01 by rational design and further studied not only optical properties but also confocal live cell imaging,and successfully employed it to monitor the alkalization process in an animal model of diabetic ulcer.More importantly,we expect our research may provide not only a novel potential tool for clinical practice to evaluate the condition of the wound bed,but also a theoretical basis for further studies on chronic wounds.