The Separation Of Evodiamine And Rutaecarpine By Three-zone Simulated Moving Bed

Evodiamine and rutaecarpine are tryptophan indole alkaloids.Evodiamine has antitumor,weight loss,and anti-Alzheimer’s disease effects.Rutaecarpine has the effects of cardiovascular relaxation,anti-cancer and immunosuppression.However,the separation of the two has always been a difficult problem.In this study,simulated moving bed technology(SMB)was used to achieve high-purity separation of evodiamine and rutaecarpine.The main findings are as follows:A method for the detection of evodiamine and rutaecarpine by high performance liquid chromatography was established.Chromatographic column:10 μm C18,4.6 mm×250 mm,mobile phase:methanol/water=80/20,detection wavelength:254 nm,flow rate:1 mL/min,injection volume,20μL,temperature:30℃.In order to achieve high purity and high efficiency separation of evodiamine and rutaecarpine,a simulated moving bed mathematical(SMB)model with temperature change was established to investigate the effect of temperature on SMB separation performance.In this study,two different particle sizes of reverse C18 was used as the stationary phase.The adsorption isotherms of evodiamine and rutaecarpine at 15,20,25,30,35,40 ℃ were measured by single-component front analysis.It was found that the adsorption amount of the 75 μm C18 stationary phase was greater.The preexperiment of the simulated moving bed found that the operating pressure of the 10 μm C18 stationary phase was too high,and it was necessary to use a lower flow rate to ensure the normal operation of the system.Finally,the 75 μm C18 chromatographic packing was selected as the stationary phase.The axial diffusion coefficient and mass transfer coefficient at the above temperature were calculated by empirical formula.The parameters were fitted to the temperature,and the henry’s constant,the axial diffusion coefficient and the mass transfer coefficient all had a good linear relationship with the temperature.The SMB and Varicol models were structured based on linear driving force model and the maximum feed amount was solved.The separation conditions were experimentally verified.At different temperatures,the separation purity of evodiamine and rutaecarpine in SMB and Varicol modes was above 99%.It was found that the lower the temperature,the higher the throughput,and the throughput of Varicol increased by 11.1%～14.9%compared with the SMB treatment at same temperatures.It showed that the three-zone Varicol process can achieve better separation performance and has good application potential.An experimental model of non-constant temperature SMB was established,and simulation calculation and experimental verification were carried out.Both examples achieved the separation purity of evodiamine and rutaecarpine over 99%.Provide a new way for SMB operation.And save energy consumption and streamline equipment,improve the utilization rate of the stationary phase,and reduce the separation cost.