| The marine ecological environment is closely related to our life.As a result of oil spills,and dangerous chemicals’ transportation,leakage and so on,it could cause serious marine pollution.Because the detections of some common marine hazardous chemicals require modern equipment support.It cannot obtain test results in a short period of time,resulting in the challenges of the rapid detection of marine hazardous chemical and emergency management.This paper aims to explore a method for hazardous chemicals which capable of rapid detection,and achieving certain sensitivity without the support of large-scale instruments,andsuitable for being equipped with rapid detection device in the unmanned surface vehicles(USV).The whole system could realize the real-time remote transmission of images,which can improve the technical support for marine emergency management.Colloidal gold test strip method has been widely used in environmental,food,medical and other analysis fields because of its rapid and convenient,coloring effect and high sensitivity.In recent years,unmanned boat technology has gradually developed towards intelligent direction,and it has been widely used in marine military,marine transportation,marine archaeology and so on.At present,information is scarce about that colloidal gold technology coupled on unmanned boats for remote real-time monitoring of hazardous chemicals.In this study,based on colloidal gold method,several rapid detection methods for typical hazardous chemicals were developed and the detection conditions were optimized.As to cyanide,a highly toxic chemical,a rapid detection method based on chemical chromogenic reaction and a device suitable for unmanned boat were developed.Finally,based on the unmanned boat technology,a remote real-time transmission scheme for the rapid detection of hazardous chemicals was designed.The main contents of this paper are as follows:(1)In this paper,antigen modifications and inductions were carried out by modifying phenylacetic acid,benzophenone(BP-3)and nitrobenzene.Corresponding antibodies were prepared by Blab/c mice.(2)Colloidal gold was synthesized and prepared by reducing chloroauric acid solution.The process of colloidal gold particles was optimized.The heating time and temperature were adjusted.The colloidal gold particles were identified by transmission electron microscopy(TEM).It was found that the required size 20 nm of the colloidal gold particles can be obtained by reduction of chloroauric acid with 1.1 ml trisodium citrate.The prepared particles are uniform in size without obvious aggregation,which met the requirements of later preparation of test paper.(3)Considering that cyanide is highly toxic,it is difficult to produce effective antibodies by antigen induction.Based on the principle of reaction discoloration between hydrocyanic acid and picric acid,the rapid detection of cyanide is explored in this paper.A rapid detection device for cyanide was established.The color reaction of picric acid test strip was studied under different temperature(50℃-80℃)and sodium carbonate dosage(5g/100ml-15g/100ml).The detection limit was reached at 0.4mg/L.In order to improve the sensitivity of picric acid strip,the device was combined with photoelectric sensor.The detection limit was improved to0.2 mg/L.(4)Using wireless communication,taking unmanned aerial vehicle as substation and mother ship as central station,selecting A9 series processors produced by ARM company,OEMV1 card as GPS module,9Xtend OEMRF module as wireless communication module and TCD1206 image sensor as video acquisition module,the framework of real-time imaging remote transmission system for unmanned aerial vehicle is designed and constructed. |
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