A Nano-drug Delivery System With Targeting Function Based On Black Phosphorous Nano-sheets In Collaboration With Photothermal And Chemotherapy For Liver Cancer

One of the main risks of liver cancer is cirrhosis caused by hepatitis.As a major country with hepatitis,the incidence of liver cancer in China remains high.But at present,we still lack effective treatment methods for liver cancer.In recent years,the rapid development of nanomedicine has made the application of nanomaterials in the field of biomedicine increasingly widespread.Among them,the use of inorganic nanoparticles as carriers,using their photothermal,photoacoustic,magnetic and other special physical and chemical properties in combination with other chemotherapeutic drugs or genetic drugs for the diagnosis and treatment of major diseases such as cancer has become a research hotspot in the field of biomedicine.In this paper,a composite nano-drug delivery system based on black phosphorus is designed.First,black phosphorous nanosheets(BPNSs)with a relatively uniform particle size were successfully prepared by an improved liquid phase exfoliation method,and their morphology,crystal structure,and photothermal characteristics were studied accordingly.The results show that the nanoplatelets have a particle size of about 200 nm and have excellent photothermal properties.Next,a functional polymer material polyethylene glycol-polyethyleneimine-folic acid(PEG-PEI-FA)was prepared by amide reaction.The appearance of characteristic peaks in infrared and proton nuclear magnetic resonance spectra indicates that the polymer is Successfully prepared.The chemotherapeutic drugs doxorubicin(DOX)and PEG-PEI-FA were loaded on the surface of BPNSs by layer-by-layer electrostatic adsorption.The increase in particle size and potential and the results of transmission electron microscopy showed that the drug delivery system was successfully prepared.The hemolysis rate of this system up to 200μg/m L is <5%,indicating that its biocompatibility is good.At the same time,in vitro cytotoxicity experiments showed that when the carrier material was as high as 100 μg/m L,the survival rates of rat hepatic stellate cells(T6),human normal hepatocytes(L02),and human umbilical vein endothelial cells(HUVEC)were 86.5%,83.8% and 85.3%respectively,indicating that it has excellent biological safety.At a low concentration of 35 μg/m L,the prepared nano-drug delivery system showed a good synergistic anti-tumor effect.In addition,cell uptake experiments show that the prepared drug delivery system has good targeting.