| Chromatographic stationary phase has always been likened to the "heart" of chromatographic separation systems,column packing materials is critical for chromatographic separation and is the key to effective separation.Therefore,in order to obtain higher separation efficiency and shorter analysis time,the development of new column packing materials has always been a long-standing research topic in chromatography technology.Carbon nanomaterials have attracted wide attention from researchers due to their unique properties.Among them,porous graphene and carbon quantum dots have superior physico and chemical properties,and as typical carbon nanomaterials,it is expected to develop into new type of chromatographic stationary phase materials.Based on the above research background,a variety of new liquid chromatography stationary phases were designed and prepared,their chromatographic performance was tested and evaluated.The main research contents are as follows:1.A porous graphene modified silica gel(Sil-PG)hydrophilic chromatographic stationary phase was prepared by modifying porous graphene onto silica gel surface.The chromatographic materials were analyzed by various characterizations such as elemental analysis,infrared(IR),transmission electron microscopy(TEM)and raman spectrum.According to the surface modification group characteristics of the chromatographic stationary phase,the selective separation of several sulfonamides was realized in the hydrophilic chromatography mode,and the effects of different chromatographic conditions on the retention of sulfonamides on the stationary phase were investigated.It has been successfully applied to the determination of sulfonamides in serum samples.By comparing the chromatographic performance with commercial hydrophilic interaction chromatography columns and bare silica gel columns,we found that the Sil-PG column exhibited better separation selectivity of sulfonamide compounds.2.Based on the first work,in order to improve the chromatographic properties of the porous graphene functionalized chromatographic stationary phase and further study the retention characteristics of porous graphene functionalized chromatographic materials.We prepared three new chromatographic stationary phases including oxidized porous graphene-modified silica stationary phase(Sil-OPG),reduced porous graphene-coated silica chromatographic stationary phase(Sil-RPG)and porous graphene modified ionic liquid-bonded silica gel chromatography stationary phase(Sil-IL-PG)by the method of oxidation,reduction and ionic liquid functionalization of porous graphene.Then the separation selectivity of these three stationary phases were investigated.Results show that these three kinds of porous graphene materials functionalized chromatographic stationary phases have significant retention behavior under different chromatographic modes,and the retention mechanism needs to be further explored.3.An ionic liquid modified carbon quantum dots functionalized silica stationary phase was prepared and further characterized by elemental analysis,TEM and IR spectroscopy.The hydrophilicity chromatographic separation performance of the novel stationary phase of 1-methylimidazole-modified quantum dots functionalized silica(Sil-Im CDs)was evaluated by separation of nucleosides and bases,sulfonamides,amino acids,saccharides and ginsenoside compounds in hydrophilic interaction chromatography(HILIC)mode.At the same time,the new stationary phase chromatographic performance were compared with the Sil-1-MIm column and the amino-modified silica column,which indicateds that the separation performance of Sil-Im CDs was significantly better than the other two stationary phases. |
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