Design,Synthesis And Application Of Novel Reaction-Based Fluorescent Probes

In recent years,fluorescence probes have become important analytical technique in current analytical methods due to their advantages of high sensitivity and selectivity,fast response,low cost.They are widely used in biological detection,environmental monitoring,food safety,disease diagnosis,and drug screening.To monitor the poison substance in food and environment,we designed and synthesized some fluorescent probes for the detection of benzoyl peroxide,bisulfite,hypochlorite（ClO^-）as well as hydrazine hydrate.This article is divided into the following five chapters:The first chapter is a literature review,which mainly describes the basic characteristics and application prospects of fluorescent probes,as well as the recent research progress of benzoyl peroxide,bisulfite,hypochlorite and hydrazine hydrate fluorescent probes.In the second chapter,a colorimetric and ratiometric fluorescent probe（Cou-BPO）based on coumarin aldehyde was designed and synthesized for the detection of benzoyl peroxide（BPO）in food.The oxidation reaction of BPO with phenylborate induces a significant fluorescent color change（from red to green）.The probe towards BPO with high selectivity and low detection limit.Cou-BPO test paper has been prepared to determine the level of BPO in food with fast response and sample.Moreover,the Cou-BPO probe was employed in cell imaging.In the third chapter,we developed a novel dual-analyte responsive fluorescent chemical sensor PTCA to monitor benzoyl peroxide and bisulfite.A single fluorescent chemical sensor can produce different fluorescence signals for the two analytes with high sensitivity,good selectivity and low detection limit.We have successfully used the probe to detect HSO₃^― and BPO in different foods with good recovery.More importantly,PTCA has been successfully developed as a test strip to visually determine HSO₃― and BPO in food samples.In the forth chapter,a dual-analytes responsive fluorescent probe PTMQ was prepared to simultaneously detect hypochlorous acid and hydrazine through different fluorescence signals.The probe showed high selectivity and sensitivity,as well as fast response.In addition,the probe has good biocompatibility and low cytotoxicity.The probe has been successfully utilized to image ClO^-and N₂H₄in living cells with different fluorescence color.In the fifth chapter,the conclusion of this paper summarizes the research content and experimental results.