Construction And Performance Of Fluorescent Probes Based On Xanthene And Cyanine Dyes

At present,the fluorescent probe method has become the preferred method for identifying and detecting important species in organisms and the environment.This method can achieve rapid,non-destructive and sensitive analysis of target test objects,and some probes can perform detection targets in specific subcellular organelles.Insitu real-time analysis,combined with fluorescent probes and fluorescent imaging technology,can monitor the redox metabolic process of living cells and biological molecules in living organisms,and is expected to be used in clinical diagnosis and pathological investigation.In order to achieve this goal,fluorescence imaging technology requires the probe to have an accurate and specific ability to detect the target analyte and also requires the probe to have the ability to image the live cell of the test object.This paper is related to human metabolic diseases.The substance is the target analyte.Several classic fluorescent probes were designed and synthesized using classic xanthene fluorescent dyes and hemicyanine dyes.The following work was mainly carried out:By introducing selenium atoms into classic fluorescein dyes,two reversible fluorescent probes Flu-Se1 and Flu-Se2 with the function of monitoring HClO/Cys redox homeostasis were designed and synthesized.The detection performance of the needle was evaluated.The analysis results show that the probe shows specific recognition for the HClO/Cys redox system.In addition to its high detection sensitivity（the detection limits are 1.1 nM/3 nM and 2.4 nM/1 nM,respectively）,the response speed is fast（<10 s）In addition to the excellent detection performance such as cyclic detection of redox process,it has also successfully realized the real-time continuous dynamic analysis of the HClO/Cys redox process of HeLa cells.The probes DER1 and DER2 with NADH recognition and detection were synthesized by the reaction of xanthene dye with 4-chloropyridine hydrochloride.For the target detection object NADH,both probes showed specific recognition performance;the use of cell fluorescence imaging technology successfully achieved HeLa cell exogenous NADH fluorescence imaging detection,which laid the foundation for the detection of cell endogenous NADH,during the imaging experiment The probe exhibits excellent biological properties such as good cell membrane permeability and low biological toxicity.Also based on the xanthene dye,the fluorescent probe Rhodol NO₂ with the specific recognition function of nitroreductase（NTR）was designed and synthesized,and the detection performance of the probe for NTR was evaluated using fluorescence spectroscopy,which laid a solid foundation for subsequent cell experiments basis.Using the semi-cyanine dye as the basic framework,the indole and the semicyanine are connected through a carbon-carbon single bond to design and synthesize a viscosity fluorescent probe Lyso-cy with lysosomal targeting localization ability.Specific testing of in vivo viscosity.Cell experiments confirmed that the probe has the ability to detect the change of lysosome viscosity of HeLa cells.