Study On The Fabrication Of A Drug Carrier Using Two-dimensional Ti₃C₂T_x Nanosheet And Its Characteristics

Recently,two-dimensional nanomaterials are attracting significant attention in the field of nano drug delivery systems,due to their high specific surface area,exceptional mechanical strength,high electrical and thermal conductivity,as well as unique optical properties.Especially,the nano drug carrier that wrapped in erythrocyte membrane has become a potential nano-bionic drug delivery technology due to its good biocompatibility and immune escape ability.Herein,a composite nano drug carrier（Ti₃C₂T_x-sp@DOX）based on titanium carbide（Ti₃C₂T_x）nanosheets is proposed,whose photothermal properties,drug loading and release characteristics,cytotoxicity and targeting properties were all studied.First,Ti₃C₂T_x nanosheets were prepared by mild etching and intercalation.Then,soybean phospholipid（SP）was modified on the surfaces of Ti₃C₂T_x,which can improve its drug-carrying capacity and protect the interior Ti₃C₂T_x from water and air to enhance the physiological stability.The obtained SPylated Ti₃C₂T_x nanosheets not only show an excellent photothermal conversion efficiency and photothermal stability,but also the ability to realase drugs upon pH or photothermal stimulations.In addition,after the erythrocyte membrane is wrapped on the surface,the prepared Ti₃C₂T_x-sp@DOX@RBCm has a good biocompatibility and immune escape ability.The CCK-8 cell viability result shows that SPylated nanosheets exhibited an excellent biocompatibility and significantly increased cytotoxicity under light irradiation.Therefore,the composite nano drug carrier based on Ti₃C₂T_x nanosheets has great potential in the fields of drug delivery and photothermal therapy.